The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Use at an assay dependent concentration. PubMed: 21573736
功能Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration.
组织特异性Widely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. Expressed in the retina by retinal pigment epithelial cells.
疾病相关Amyotrophic lateral sclerosis 16, juvenile
序列相似性Belongs to the ERG2 family.
细胞定位Nucleus inner membrane. Nucleus outer membrane. Endoplasmic reticulum membrane. Lipid droplet. Cell junction. Cell membrane. Cell projection > growth cone. Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum. Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding.
All lanes : Anti-OPRS1 antibody (ab53852) at 2 µg/ml
Lane 1 : mouse brain homogenate Lane 2 : rat liver homogenate Lane 3 : rat brain homogenate
Predicted band size : 25 kDa Observed band size : 25 kDa Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry - Free Floating - Anti-OPRS1 antibody (ab53852)This image is courtesy of an Abreview submitted by Marco Peviani.
ab53852 staining OPRS1 in Lumbar Spinal Cord tissue (30 micron stections) by Immunohistochemistry (Free-floating sections). Blocking was done by incubating the sections for 1 hour at room temperature in NGS 10% + Triton 0.1% in PBS. The primary antibody was diluted 1/250 in 3% Goat serum in PBS and incubated with the sample for 16 hours at 4°C. An Alexa fluor® 647-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500. Sections were also immunostained with Hoechst dye (blue) to highlight nuclei. The green signal is GFP expressed in motor neuronal cells.
Omi T et al. Fluvoxamine alleviates ER stress via induction of Sigma-1 receptor. Cell Death Dis5:e1332 (2014).
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Szabo A et al. Psychedelic N,N-Dimethyltryptamine and 5-Methoxy-N,N-Dimethyltryptamine Modulate Innate and Adaptive Inflammatory Responses through the Sigma-1 Receptor of Human Monocyte-Derived Dendritic Cells. PLoS One9:e106533 (2014).
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