Anti-OPCML抗体(ab100923)

概述

  • 产品名称Anti-OPCML抗体
    参阅全部 OPCML 一抗
  • 描述
    兔多克隆抗体to OPCML
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 75-341 of Human OPCML (BC117254).

  • 阳性对照
    • WB: Human fetal brain lysate and HeLa cell lysate IHC-P: Human fetal brain tissue IF/ICC: PC12 cell line.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab100923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能Binds opioids in the presence of acidic lipids; probably involved in cell contact.
  • 疾病相关Ovarian cancer
  • 序列相似性Belongs to the immunoglobulin superfamily. IgLON family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GM181 antibody
    • IgLON family member 1 antibody
    • IGLON1 antibody
    • OBCAM antibody
    • OPCM antibody
    • OPCM_HUMAN antibody
    • OPCML antibody
    • Opiate binding cell adhesion molecule antibody
    • Opioid binding cell adhesion molecule antibody
    • Opioid binding protein/cell adhesion molecule antibody
    • Opioid binding protein/cell adhesion molecule-like antibody
    • Opioid binding protein/cell adhesion molecule-like preprotein antibody
    • Opioid-binding cell adhesion molecule antibody
    • Opioid-binding protein/cell adhesion molecule antibody
    see all

Anti-OPCML antibody 图像

  • ICC/IF image of ab100923 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100923, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

     

  • All lanes : Anti-OPCML antibody (ab100923) at 1/500 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 38 kDa
  • ab100923, at a 1/100 dilution, staining OPCML in formalin fixed, paraffin embedded Human fetal brain tissue by Immunohistochemistry.

Anti-OPCML antibody (ab100923)参考文献

ab100923 has not yet been referenced specifically in any publications.

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