antigen: PLSEPSRSST RPPSIADPDP SDLPVDRAAT KAPGMEPSGS VAGLGELDPG AFLDKDAECR EELLKDDSSE HGAPDSKEKT PGRHRRFTGD, corresponding to amino acids 160-249 of Human OPA1L according to Q9NX94.
- Human gall bladder tissue, transfected HEK293T cells overexpressing OPA1L with a myc-DDK tag.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1L antibody (ab122401)
ab122401, at a 1/100 dilution, staining OPA1L in glandular cells of paraffin-embedded Human gall bladder tissue by immunohistochemistry.
Western blot - Anti-OPA1L antibody (ab122401)
All lanes : Anti-OPA1L antibody (ab122401) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : Lysate from HEK293T cells overexpressing OPA1L with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"