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Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Rat OPA1.
(Peptide available as ab42363.)
Our Abpromise guarantee covers the use of ab42364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/200 - 1/500.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 92,86 kDa (predicted molecular weight: 111 kDa).
Abcam recommends using milk as the blocking agent.
|IP||Use at an assay dependent concentration.|
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
OPA1 was immunoprecipitated using 0.5mg Rat Brain whole tissue lysate, 5µg of Rabbit polyclonal to OPA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, rat brain whole tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab42364.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 92kDa: OPA1; non specific - 100kDa: We are unsure as to the identity of this extra band.