概述

  • 产品名称Anti-ONECUT3抗体
    参阅全部 ONECUT3 一抗
  • 描述
    兔多克隆抗体to ONECUT3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: LAACKRKEQE QQKERALQPK KQRLVFTDLQ RRTLIAIFKE NKRPSKEMQV, corresponding to amino acids 396-445 of Human ONECUT3

  • 阳性对照
    • HepG2 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab55912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

靶标

Anti-ONECUT3 antibody 图像

  • Anti-ONECUT3 antibody (ab55912) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

Anti-ONECUT3 antibody (ab55912)参考文献

ab55912 has not yet been referenced specifically in any publications.

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