概述

  • 产品名称Anti-Oncostatin M抗体
    参阅全部 Oncostatin M 一抗
  • 描述
    兔多克隆抗体to Oncostatin M
  • 经测试应用适用于: WB, ELISA, Neutralisingmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hOncostatin-M (human Oncostatin-M).

性能

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应用

Our Abpromise guarantee covers the use of ab9633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
Neutralising
  • 应用说明Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hOncostatin-M (10 ng/ml), a concentration of 0.1 - 0.175 µg/ml of this antibody is required

    ELISA:To detect hOncostatin-M by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hOncostatin-M.

    Western Blot:To detect hOncostatin-M by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin-M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • 靶标

    • 功能Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST).
    • 序列相似性Belongs to the LIF/OSM family.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MGC20461 antibody
      • ONCM_HUMAN antibody
      • Oncostatin M antibody
      • Oncostatin-M antibody
      • OSM antibody
      see all

    Anti-Oncostatin M antibody (ab9633)参考文献

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    For the detection antibody you can use a biotinylated secondary such as ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin))-one of the compatible secondaries for ab9633 listed at the bottom of the datasheet.

    Thank you for your enquiry. Below is a general Direct ELISA protocol which should be helpful for you. If you have any more questions, please contact us again. Direct ELISA: To detect hOncostatin-M by direct ELISA (using 100µl/well antibody solution)...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"