概述

  • 产品名称Anti-OMA1抗体
    参阅全部 OMA1 一抗
  • 描述
    兔多克隆抗体to OMA1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide designed within residues: WAICPRDSLA LLCQWIQSKL QEYMFNRPYS RKLEAEADKI GLLLAAKACA, corresponding to internal sequence amino acids 359 - 408 of Human OMA1 (NP_660286).

  • 阳性对照
    • 721_B cell line lysate IF: HeLa cell line

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab104316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.Can be blocked with OMA1 peptide (ab129125). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Mitochondrial protease.
  • 组织特异性Widely expressed, with strong expression in the heart, skeletal muscle, kidney and liver.
  • 序列相似性Belongs to the peptidase M48 family.
  • 细胞定位Mitochondrion membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2010001O09Rik antibody
    • DAB1 antibody
    • FLJ33782 antibody
    • Metalloendopeptidase OMA1 antibody
    • Metalloendopeptidase OMA1, mitochondrial antibody
    • Metalloprotease-related protein 1 antibody
    • mitochondrial antibody
    • MPRP 1 antibody
    • MPRP-1 antibody
    • MPRP1 antibody
    • OMA1 antibody
    • OMA1 homolog zinc metallopeptidase antibody
    • OMA1 homolog, zinc metallopeptidase (S. cerevisiae) antibody
    • OMA1 zinc metallopeptidase antibody
    • OMA1 zinc metallopeptidase homolog antibody
    • OMA1_HUMAN antibody
    • Overlapping activity with M-AAA protease antibody
    • Overlapping with the m-AAA protease 1 homolog antibody
    • peptidase antibody
    • YKR087C antibody
    • Zinc metallopeptidase OMA1 antibody
    • ZMPOMA1 antibody
    see all

Anti-OMA1 antibody 图像

  • Anti-OMA1 antibody (ab104316) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size : 60 kDa
  • ICC/IF image of ab104316 stained HeLa cells. The cells were 4% paraformaldehyde fixed ( min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104316, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-OMA1 antibody (ab104316)参考文献

This product has been referenced in:
  • Kong B  et al. p53 Is Required for Cisplatin-induced Processing of the Mitochondrial Fusion Protein L-Opa1 That Is Mediated by the Mitochondrial Metallopeptidase Oma1 in Gynecologic Cancers. J Biol Chem 289:27134-45 (2014). Human . Read more (PubMed: 25112877) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rabbit Tissue lysate - whole (aorta)
Specification aorta
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 7.5% · Temperature: 22°C
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提交于 Oct 29 2013

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (8-10%)
Sample Rat Cell lysate - whole cell (neurons)
Specification neurons
Treatment control , oxgen glucose deprivation
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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提交于 Oct 08 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (MEFs)
Specification MEFs
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Oct 30 2013

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% GEL)
Sample Human Cell lysate - other (HELA)
Specification HELA
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Oct 30 2013

Application Western blot
Sample Rat Cell lysate - whole cell (Heart fibroblasts)
Loading amount 10 µg
Specification Heart fibroblasts
Gel Running Conditions Reduced Denaturing (10% polyacrylamide)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Eli Anuka

Verified customer

提交于 Jun 08 2012

Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount 10 µg
Specification 3T3 cell line
Gel Running Conditions Reduced Denaturing (10% polyacrylamide)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Eli Anuka

Verified customer

提交于 Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cell line)
Loading amount 10 µg
Specification HeLa cell line
Gel Running Conditions Reduced Denaturing (10% polyacrylamide)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Eli Anuka

Verified customer

提交于 Jun 07 2012

Thank you for contacting us.
The anti-OMA1 antibody (ab104316) is raised against a peptide taken from within the region of 359-408 of human OMA1. This shares 89% homology with the rat OMA1 protein and we would therefore predict that the antibody w...

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Thank you for contacting us. The blocking peptide for ab104316 has been added to our catalog as ab129125. I have submitted a product suggestion to make a mouse monocolonal antibody to OMA1, but it may take several months to produce one. I hope this hel...

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Thank you for sending the image of your blot.


According to the UniProt Database, there are two isoforms of OMA1. These isoforms will run around 55 & 60 kDa. The protein also has a 13 amino acid transit peptide, which will slightly l...

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