Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody (ab53041)This image is courtesy of an Abreview submitted by Carl Hobbs
ab53041 staining cat brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53041 at 1/500 in TRIS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Oligodendrocyte Specific Protein antibody (ab53041)Image courtesy of an anonymous Abreview.
ab53041 staining Oligodendrocyte Specific Protein in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in ice-cold methanol and then blocked using 20% serum with 0.5% Saponin for 1 hour at 4°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody (ab53041)This image is courtesy of an Abreview submitted by Dr J Moffett
ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C. A biotinylated goat anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody (ab53041)This image is a courtesy of Anonymous Abreview
ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used at dilution 1/1000 as secondary antibody. The image was taken from the cortex of an adult mouse, near the midline.
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