Anti-Oligodendrocyte Specific Protein抗体(ab53041)

概述

  • 产品名称
    Anti-Oligodendrocyte Specific Protein抗体
    参阅全部 Oligodendrocyte Specific Protein 一抗
  • 描述
    兔多克隆抗体to Oligodendrocyte Specific Protein
  • 特异性
    Detects endogenous levels of total Oligodendrocyte Specific Protein
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, WB, ELISA, IHC-P, IHC (PFA fixed)more details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Goat, Chicken, Cow, Cat, Human, Common marmoset
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Mouse brain extract

性能

应用

Our Abpromise guarantee covers the use of ab53041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/5000.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC (PFA fixed) 1/3000.

靶标

图片

  • All lanes : Anti-Oligodendrocyte Specific Protein antibody (ab53041) at 1/500 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Mouse brain extract with peptide


    Predicted band size : 22 kDa
    Observed band size : 22 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

  • ab53041 staining Oligodendrocyte Specific Protein in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in ice-cold methanol and then blocked using 20% serum with 0.5% Saponin for 1 hour at 4°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. DAPI was used to stain the cell nuclei (blue).

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  • ab53041 staining cat brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53041 at 1/500 in TRIS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody was used as the secondary antibody.

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  • ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

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  • ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used at dilution 1/1000 as secondary antibody. The image was taken from the cortex of an adult mouse, near the midline.

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文献

This product has been referenced in:
  • Appu AP  et al. Increasing N-acetylaspartate in the Brain during Postnatal Myelination Does Not Cause the CNS Pathologies of Canavan Disease. Front Mol Neurosci 10:161 (2017). IHC ; Mouse . Read more (PubMed: 28626388) »
  • Luo L  et al. Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats. Exp Ther Med 13:645-650 (2017). Read more (PubMed: 28352345) »

See all 24 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 1 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 May 24 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (In vitro differentiated mouse neural stem cells)
Permeabilization
No
Specification
In vitro differentiated mouse neural stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Apr 30 2015

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Cultured Cells (engineered nervous tissue)
Specification
engineered nervous tissue
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 20 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification
Hippocampal homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 01 2014

Application
IHC - Wholemount
Sample
Zebrafish Embryo (48hpf oligodendrocyte/motoneurons)
Specification
48hpf oligodendrocyte/motoneurons
Username

Abcam user community

Verified customer

提交于 Mar 05 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Lizard Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jun 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (testis)
Specification
testis
Fixative
bouin's solution
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain (cortex))
Specification
mouse brain (cortex)
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Triton during block and staining
Blocking step
PBS, 10%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Feb 08 2013

Thank you for contacting us.

Could you please clarify which publication you are referring to? We have four different references listed on the datasheet for ab53041.

While the O4 clone is a very well known oligodendrocyte marker, it ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 03 2012

1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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