Anti-Olig3抗体(ab78006)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Olig3
  • 经测试应用
    适用于: IHC-Fr, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 53-66 of Human Olig3

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Constituents: 50% Ammonium sulphate, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Antibody is affinity purified but presented as an ammonium sulfate precipitated purified material for stability.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab78006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
WB
ICC
  • 应用说明
    ICC: 1/200 - 1/1000.
    IHC-Fr: 1/200 - 1/1000 for 90 minutes. Cut 10um slices on a cryostat and dry slides at RT for 30-90 minutes. Fix with cold Acetone/Methanol (50/50) for 2 minutes or alternatively with 4% PFA followed by treatment with a 0.05% trypsin solution (incubate for 10 minutes at 37°C, then wash 3 times with PBS)
    WB: 1/200 - 1/1000. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
    • 序列相似性
      Contains 1 bHLH (basic helix-loop-helix) domain.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Basic helix loop helix domain containing class B protein 7 antibody
      • Basic helix-loop-helix domain-containing protein, class B, 7 antibody
      • bHLHB7 antibody
      • bHLHe20 antibody
      • Class B basic helix loop helix protein 7 antibody
      • Class B basic helix-loop-helix protein 7 antibody
      • Class E basic helix loop helix protein 20 antibody
      • Class E basic helix-loop-helix protein 20 antibody
      • Olig3 antibody
      • OLIG3_HUMAN antibody
      • Oligo3 antibody
      • Oligodendrocyte lineage transcription factor 3 antibody
      • Oligodendrocyte specific bHLH transcription factor 3 antibody
      • Oligodendrocyte transcription factor 3 antibody
      see all

    图片

    • Confocal IF analysis of PC12 celss using ab78006 (Red). Actin filaments have been labeled with Alexa Fluor 488 - Phalloidin (Green). Nucleus is stained with DAPI (Blue).


    • Predicted band size : 29 kDa

    文献

    ab78006 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Vielen Dank für Ihre Anfrage.

    Zwar haben wir noch keine Publikation für diesen Antikörper in IHC, haben diesen Antikörper aber bisher erfolgreich in Kyrosektionen getestet, und können Ihnen daher fuer diese Applikation unsere Garantie in der...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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