Anti-Olig2抗体(ab56643)

概述

性能

应用

Our Abpromise guarantee covers the use of ab56643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 5 µg/ml. For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Olig2 (ab77953)ab> as Detection.

靶标

  • 功能Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
  • 组织特异性Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
  • 疾病相关Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 结构域The bHLH is essential for interaction with NKX2-2.
  • 细胞定位Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic domain helix loop helix protein class B 1 antibody
    • Basic helix loop helix protein class B 1 antibody
    • BHLHB antibody
    • bHLHB1 antibody
    • bHLHe19 antibody
    • Class B basic helix loop helix protein 1 antibody
    • Class B basic helix-loop-helix protein 1 antibody
    • class E basic helix loop helix protein 19 antibody
    • Class E basic helix-loop-helix protein 19 antibody
    • Human protein kinase C binding protein RACK17 antibody
    • Olig2 antibody
    • OLIG2_HUMAN antibody
    • Oligo2 antibody
    • Oligodendrocyte lineage transcription factor 2 antibody
    • Oligodendrocyte specific bHLH transcription factor 2 antibody
    • Oligodendrocyte transcription factor 2 antibody
    • OTTHUMP00000067569 antibody
    • OTTHUMP00000067570 antibody
    • PRKCBP2 antibody
    • Protein kinase C binding protein 2 antibody
    • Protein kinase C binding protein RACK17 antibody
    • Protein kinase C-binding protein 2 antibody
    • Protein kinase C-binding protein RACK17 antibody
    • RACK17 antibody
    see all

Anti-Olig2 antibody 图像

  • Standard Curve for Olig2; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal to Olig2 (ab56643) at 5 ug/ml and Detector Antibody Rabbit polyclonal to Olig2 (ab77953) at 0.5 ug/ml.
  • Western blot against tagged recombinant protein immunogen using ab56643 Olig2 antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa
  • IHC image of ab56643 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56643, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

实验方案

Anti-Olig2 antibody (ab56643)参考文献

ab56643 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA fixed)
Sample Mouse Tissue sections (brain)
Specification brain
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提交于 Jul 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"