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A region within synthetic peptide: SLPGSGLPSV GSIRPPHGLL KSPSAAAAAP LGGGGGGSGA SGGFQHWGGM, corresponding to amino acids 242-291 of Olig2
Our Abpromise guarantee covers the use of ab42453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human Olig2 peptide (ab111666). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20025521|
|IHC-Fr||1/200. (see Abreview and notes)|
|IHC (PFA fixed)||1/1000.|