ab75882 detects endogenous levels of total OLFML2A protein.
Synthesized peptide derived from internal of human OLFML2A.
Extracts from RAW264.7 cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
1/500 - 1/1000.
Use at an assay dependent concentration.
OLFML2A contains one olfactomedin-like domain. Its exact function is unknown. There are three named isoforms produced by alternative splicing.
Olfactomedin like 2A antibody
Olfactomedin like protein 2A antibody
Immunohistochemistry (Frozen sections) - Anti-OLFML2A antibody (ab75882) This image is courtesy of an Abreview submitted by Iram Pablo Rodriguez-Sanchez
ab75882 staining OLFML2A in Human retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 12 hours at 25°C. Samples were incubated with primary antibody (1/500) for 2 hours at 25°C. A Cy3®-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Western blot - OLFML2A antibody (ab75882)
All lanes : Anti-OLFML2A antibody (ab75882) at 1/500 dilution Lane 1 : extracts from RAW264.7 cells Lane 2 : extracts from RAW264.7 cells with immunizing peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 73 kDa Observed band size : 73 kDa
Immunocytochemistry/ Immunofluorescence - OLFML2A antibody (ab75882)
ab75882, at a dilution of 1/500-1/1000, staining OLFML2A in A549 cells by Immunofluorescence with (+) or without (-) immunising peptide.
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