Anti-OLFML2A抗体(ab75882)

概述

  • 产品名称Anti-OLFML2A抗体
    参阅全部 OLFML2A 一抗
  • 描述
    兔多克隆抗体to OLFML2A
  • 特异性ab75882 detects endogenous levels of total OLFML2A protein.
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthesized peptide derived from internal of human OLFML2A.

  • 阳性对照
    • Extracts from RAW264.7 cells A549 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性OLFML2A contains one olfactomedin-like domain. Its exact function is unknown. There are three named isoforms produced by alternative splicing.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • FLJ00237 antibody
    • Olfactomedin like 2A antibody
    • Olfactomedin like protein 2A antibody
    • OLM2A_HUMAN antibody
    • Photomedin 1 antibody
    • PRO34319 antibody
    • UNQ9394/PRO34319 antibody
    see all

Anti-OLFML2A antibody 图像

  • ab75882 staining OLFML2A in Human retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 12 hours at 25°C. Samples were incubated with primary antibody (1/500) for 2 hours at 25°C. A Cy3®-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-OLFML2A antibody (ab75882) at 1/500 dilution

    Lane 1 : extracts from RAW264.7 cells
    Lane 2 : extracts from RAW264.7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • ab75882, at a dilution of 1/500-1/1000, staining OLFML2A in A549 cells by Immunofluorescence with (+) or without (-) immunising peptide.

Anti-OLFML2A antibody (ab75882)参考文献

ab75882 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Retina)
Specification Retina
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
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Prof. IRAM PABLO RODRIGUEZ-SANCHEZ

Verified customer

提交于 Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"