概述

  • 产品名称Anti-OLFM4抗体
    参阅全部 OLFM4 一抗
  • 描述
    兔多克隆抗体to OLFM4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chimpanzee
  • 免疫原

    Synthetic peptide:

    YVYNDGYLLNYDLSVLQKPQ

    , corresponding to amino acids 400-419 of Human OLFM4 (Gen Bank. gb|AAH47740.1).

  • 阳性对照
    • Human liver lysate or colon tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 3 µg/ml. Predicted molecular weight: 57 kDa.

Load no more than 25ug of protein in WB.

IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion.
  • 组织特异性Expressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues.
  • 序列相似性Contains 1 olfactomedin-like domain.
  • 结构域The olfactomedin-like domain is involved in the interaction with cadherin.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Secreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antiapoptotic protein GW112 antibody
    • bA209J19.1 antibody
    • G-CSF-stimulated clone 1 protein antibody
    • GC1 antibody
    • GW112 antibody
    • hGC 1 antibody
    • hGC-1 antibody
    • hOLfD antibody
    • KIAA4294 antibody
    • olfactomedin 4 antibody
    • Olfactomedin-4 antibody
    • OlfD antibody
    • OLFM4 antibody
    • OLFM4_HUMAN antibody
    • OLM4 antibody
    • UNQ362 antibody
    see all

Anti-OLFM4 antibody 图像

  • All lanes : Anti-OLFM4 antibody (ab96280) at 2 µg/ml

    Lane 1 : Human liver lysate
    Lane 2 : Human liver lysate with immunizing peptide


    Predicted band size : 57 kDa
  • Immunohistochemical analysis of OLFM4 in formalin-fixed, paraffin-embedded Human colon tissue using an isotype control (top) and ab96280 (bottom) at 5 ug/ml.

Anti-OLFM4 antibody (ab96280)参考文献

This product has been referenced in:
  • Amirbeagi F  et al. Olfactomedin-4 autoantibodies give unusual c-ANCA staining patterns with reactivity to a subpopulation of neutrophils. J Leukoc Biol 97:181-9 (2015). Read more (PubMed: 25387833) »
  • Giammanco A  et al. Intestinal epithelial HuR modulates distinct pathways of proliferation and apoptosis and attenuates small intestinal and colonic tumor development. Cancer Res 74:5322-35 (2014). Mouse . Read more (PubMed: 25085247) »

See all 4 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Mouse Tissue lysate - whole (Spleen, liver, brian, vagina)
Specification Spleen, liver, brian, vagina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. Bareza Rasoul

Verified customer

提交于 Mar 27 2014

I have issued a free of charge replacement.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (intestine)
Specification intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 16 2013

Code de remise : xxxxxxxxx
Date d’expiration : 29/03/13

Je suis ravie que vous souhaitiez participer a notre offre et tester ab96280 chez la souris. Le code de réduction correspondant est xxxxxxxx. Ce code de réducti...

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Merci de nous avoir contactés.

Je suis désolée qu’ab85046 soit en rupture de stock.

Je vous propose ab96280 (http://www.abcam.com/ab96280) qui a été testé en immunohistochemie sur coup...

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Thank you for your email. I have sent the lysates ab29889 with order number 1007989. I hope this will help.

I have now received a reply from lab. They have confirmed observing a 57kDa using human, rat and mouse liver extracts and 57 kD/ 40 kD band in the stomach extracts. I am OK for sending positive control lysates. However I would be interested in getting ...

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Thank you for your email. I have forwarded the data to my colleague in lab and now I am waiting a reply. I will contact you again soon; normally they write back to us within 24 hours. I have also done further search which I would be happy in sharing wi...

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Thanks you very much for contacting us. My colleagues has just forwarded your details to me. I am sorry to hear that you are observing multiple bands again. I will certainly look into this case further and would also like to discuss this case with l...

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Thank you for contacting us. We normally use 10% normal serum in 1%BSA and TBS for 1-2 hours RT. I would also recomend blcoking endogenous peroxidases if HRP detection system is being used.   I hope this information is helpful to you. Please do...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"