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Our Abpromise guarantee covers the use of ab96280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 3 µg/ml. Predicted molecular weight: 57 kDa.
Load no more than 25ug of protein in WB.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|