Anti-Olfactory Marker Protein抗体(ab87338)

概述

  • 产品名称Anti-Olfactory Marker Protein抗体
    参阅全部 Olfactory Marker Protein 一抗
  • 描述
    兔多克隆抗体to Olfactory Marker Protein
  • 经测试应用适用于: WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Goat, Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Olfactory Marker Protein.

  • 阳性对照
    • This antibody gave a positive signal in Mouse and Rat Olfactory Bulb Tissue Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab87338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 mg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
IHC-FoFr 1/1000.

靶标

  • 相关性Olfactory marker protein (OMP) is an abundant, 19kDa, cytosolic protein that is almost exclusively expressed in mature, functioning, olfactory neurons but not in the neural precursor basal cells. The OMP gene structure and protein sequence are highly conserved between mouse, rat and human. Its tissue specific expression in the receptor cells together with the results of the mouse knockout studies show that OMP represents a novel modulatory component of the odor detection/signal transduction cascade.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • Olfactory marker protein antibody
    • Olfactory neuronal specific protein antibody
    • OMP antibody

Anti-Olfactory Marker Protein antibody 图像

  • IHC-FoFr image of Olfactory Marker Protein staining on mouse olfactor bulb using ab87338. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat.

    See Abreview

  • Anti-Olfactory Marker Protein antibody (ab87338) at 1 µg/ml + Rat Olfactory Bulb Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • Anti-Olfactory Marker Protein antibody (ab87338) at 1 µg/ml + Mouse Olfactory Bulb Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa


    Exposure time : 16 minutes

Anti-Olfactory Marker Protein antibody (ab87338)参考文献

This product has been referenced in:
  • Chandler JD  et al. Low-dose oral cadmium increases airway reactivity and lung neuronal gene expression in mice. Physiol Rep 4:N/A (2016). WB ; Mouse . Read more (PubMed: 27401458) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (Olfactory epithelium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako citrate target retrieval buffer
Permeabilization No
Specification Olfactory epithelium
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Olfactory Epithelium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako citrate antigen retrieval buffer
Permeabilization No
Specification Olfactory Epithelium
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 16 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain - Olfactory Bulb)
Specification Brain - Olfactory Bulb
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 20 2012

We use 5% BSA for blocking and diluting the primary antibody, which for some antibodies will give a stronger signal than 5% milk. So you may want to try that switch, if you are using milk to block and dilute. I also suggest simply using more antibody (...

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