Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体(ab96718)

概述

  • 产品名称
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体
    参阅全部 OGT / O-Linked N-Acetylglucosamine Transferase 一抗
  • 描述
    兔多克隆抗体to OGT / O-Linked N-Acetylglucosamine Transferase
  • 经测试应用
    适用于: WB, IHC-P, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human, Zebrafish
    预测可用于: Rat, Cow, Pig
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 213 and 462 of O-Linked N-Acetylglucosamine Transferase

  • 阳性对照
    • 293T, H1299, HeLa, Molt-4 and Raji cell lysates. Cal27 xenograft. IF: DU145 cell line

性能

应用

Our Abpromise guarantee covers the use of ab96718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 117 kDa.
IHC-P 1/100 - 1/250.
IP 1/100 - 1/500.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能
    Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
  • 组织特异性
    Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
  • 通路
    Protein modification; protein glycosylation.
  • 序列相似性
    Belongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.
  • 结构域
    The TPR repeat domain mediates recognition of protein substrates.
  • 翻译后修饰
    Ubiquitinated, leading to its proteasomal degradation.
  • 细胞定位
    Cytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody 图像

  • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 117 kDa
    7.5% SDS PAGE
  • All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 117 kDa
    Observed band size : 117 kDa


    Exposure time : 5 minutes
  • Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using ab96718 at 1/100 dilution.
  • Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5µg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody. 

  • Ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF  (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1:500 dilution.

  • ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)参考文献

This product has been referenced in:
  • Kubota Y  et al. WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins. PLoS One 12:e0180714 (2017). Human . Read more (PubMed: 28686627) »
  • Ding X  et al. Mixed Lineage Leukemia 5 (MLL5) Protein Stability Is Cooperatively Regulated by O-GlcNac Transferase (OGT) and Ubiquitin Specific Protease 7 (USP7). PLoS One 10:e0145023 (2015). IF, IHC ; Human . Read more (PubMed: 26678539) »

See all 6 Publications for this product

Product Wall

Application
Western blot
Sample
Drosophila melanogaster Cell lysate - whole cell (Whole cell lysate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
10 µg
Specification
Whole cell lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (mast cell type)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
10 µg
Specification
mast cell type
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Seong Hoon Kim

Verified customer

提交于 May 24 2016

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (T lymphocyte (human))
Specification
T lymphocyte (human)
Treatment
0.5, 1 mM calpeptin for 15min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Feb 12 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (T lymphocyte)
Specification
T lymphocyte
Treatment
pathogen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 May 20 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (T lymphocyte)
Specification
T lymphocyte
Treatment
pathogen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse kidney sections)
Specification
mouse kidney sections
Fixative
formalin overnight, ethanol and wax
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Mar 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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