Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
Protein modification; protein glycosylation.
Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
The TPR repeat domain mediates recognition of protein substrates.
Ubiquitinated, leading to its proteasomal degradation.
Western blot - O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size : 117 kDa 7.5% SDS PAGE
Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/ml
Lane 1 : Marker Lane 2 : Zebrafish brain homogenate at 20 µg Lane 3 : Zebrafish heart homogenate at 20 µg Lane 4 : Zebrafish liver homogenate at 20 µg Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 117 kDa Observed band size : 117 kDa
Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5µg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody.
Ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1:500 dilution.
ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Kubota Y et al. WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins. PLoS One12:e0180714 (2017).
Read more (PubMed: 28686627) »
Ding X et al.
Mixed Lineage Leukemia 5 (MLL5) Protein Stability Is Cooperatively Regulated by O-GlcNac Transferase (OGT) and Ubiquitin Specific Protease 7 (USP7).
PLoS One10:e0145023 (2015).
Read more (PubMed: 26678539) »