The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
Protein modification; protein glycosylation.
Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
The TPR repeat domain mediates recognition of protein substrates.
Ubiquitinated, leading to its proteasomal degradation.
Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One9:e83437 (2014).
Read more (PubMed: 24465380) »
Andrés-Bergós J et al. The increase in o-linked N-acetylglucosamine protein modification stimulates chondrogenic differentiation both in vitro and in vivo. J Biol Chem287:33615-28 (2012).
Read more (PubMed: 22859309) »