The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
组织特异性Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
通路Protein modification; protein glycosylation.
序列相似性Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
结构域The TPR repeat domain mediates recognition of protein substrates.
翻译后修饰Ubiquitinated, leading to its proteasomal degradation.
Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One9:e83437 (2014).
Read more (PubMed: 24465380) »
Andrés-Bergós J et al. The increase in o-linked N-acetylglucosamine protein modification stimulates chondrogenic differentiation both in vitro and in vivo. J Biol Chem287:33615-28 (2012).
Read more (PubMed: 22859309) »