The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at a concentration of 2.5 - 5 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).
Suitable for Array.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
组织特异性Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
通路Protein modification; protein glycosylation.
序列相似性Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
结构域The TPR repeat domain mediates recognition of protein substrates.
翻译后修饰Ubiquitinated, leading to its proteasomal degradation.