The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa). A 78kDa band may be seen in some preparations.
Use a concentration of 3 - 6 µg/ml.
功能Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
组织特异性Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
通路Protein modification; protein glycosylation.
序列相似性Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
结构域The TPR repeat domain mediates recognition of protein substrates.
翻译后修饰Ubiquitinated, leading to its proteasomal degradation.
Cox EJ & Marsh SA Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart. Cardiovasc Diabetol12:101 (2013).
Read more (PubMed: 23835259) »
Kebede M et al. Glucose activates free fatty acid receptor 1 gene transcription via phosphatidylinositol-3-kinase-dependent O-GlcNAcylation of pancreas-duodenum homeobox-1. Proc Natl Acad Sci U S A109:2376-81 (2012).
Read more (PubMed: 22308370) »