Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体(ab50270)

概述

  • 产品名称
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体
    参阅全部 OGT / O-Linked N-Acetylglucosamine Transferase 一抗
  • 描述
    兔多克隆抗体to OGT / O-Linked N-Acetylglucosamine Transferase
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide:

    WEHYAAGNKPDHMI

    conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to amino acids 1024-1037 of Human O-Linked N-Acetylglucosamine

  • 阳性对照
    • HeLa cell nuclear extract and A549 human lung carcinoma cells.

性能

应用

Our Abpromise guarantee covers the use of ab50270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).
ICC/IF 1/50 - 1/100. (Cells fixed with paraformaldehyde/triton).

靶标

  • 功能
    Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
  • 组织特异性
    Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
  • 通路
    Protein modification; protein glycosylation.
  • 序列相似性
    Belongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.
  • 结构域
    The TPR repeat domain mediates recognition of protein substrates.
  • 翻译后修饰
    Ubiquitinated, leading to its proteasomal degradation.
  • 细胞定位
    Cytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

图片

  • Cytoplasmic, and nuclear localization of O-Linked N-Acetylglucosamine in human lung carcinoma (A549) cells stained by ab50270. A. Working dilution of 1:100 B. Working dilution of 1:50

文献

This product has been referenced in:
  • Runager K  et al. Targeting O-glycosyltransferase (OGT) to promote healing of diabetic skin wounds. J Biol Chem 289:5462-6 (2014). WB ; Human . Read more (PubMed: 24398691) »
  • Li MD  et al. O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination. Cell Metab 17:303-10 (2013). Read more (PubMed: 23395176) »

See all 3 Publications for this product

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