Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体(ab50270)
- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
- 产品名称Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体
参阅全部 OGT / O-Linked N-Acetylglucosamine Transferase 一抗 - 描述兔多克隆抗体to OGT / O-Linked N-Acetylglucosamine Transferase
- 经测试应用适用于: WB, ICC/IFmore details
- 种属反应性与反应: Mouse, Rat, Human
预测可用于: Pig
- 免疫原
Synthetic peptide:
WEHYAAGNKPDHMI
conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to amino acids 1024-1037 of Human O-Linked N-Acetylglucosamine - 阳性对照
- HeLa cell nuclear extract and A549 human lung carcinoma cells.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 -
Concentration information loading... - 纯度IgG fraction
- 纯化说明Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab50270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa). | |
| ICC/IF | 1/50 - 1/100. (Cells fixed with paraformaldehyde/triton). |
靶标
- 功能Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
- 组织特异性Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
- 通路Protein modification; protein glycosylation.
- 序列相似性Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. - 结构域The TPR repeat domain mediates recognition of protein substrates.
- 翻译后修饰Ubiquitinated, leading to its proteasomal degradation.
- 细胞定位Cytoplasm. Nucleus. Mostly in the nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 26295 Rat
- Omim: 300255 Human
- SwissProt: O15294 Human
- SwissProt: Q8CGY8 Mouse
- SwissProt: Q27HV0 Pig
see all -
别名
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody 图像
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Immunocytochemistry/ Immunofluorescence - O-Linked N-Acetylglucosamine antibody (ab50270)Cytoplasmic, and nuclear localization of O-Linked N-Acetylglucosamine in human lung carcinoma (A549) cells stained by ab50270. A. Working dilution of 1:100 B. Working dilution of 1:50
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab50270)参考文献
This product has been referenced in:
- Runager K et al. Targeting O-glycosyltransferase (OGT) to promote healing of diabetic skin wounds. J Biol Chem 289:5462-6 (2014). WB ; Human . Read more (PubMed: 24398691) »
- Rogacka D et al. Expression of GFAT1 and OGT in podocytes: transport of glucosamine and the implications for glucose uptake into these cells. J Cell Physiol 225:577-84 (2010). WB, ICC/IF ; Rat . Read more (PubMed: 20506529) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"