The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).
1/50 - 1/100. (Cells fixed with paraformaldehyde/triton).
功能Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
组织特异性Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
通路Protein modification; protein glycosylation.
序列相似性Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
结构域The TPR repeat domain mediates recognition of protein substrates.
翻译后修饰Ubiquitinated, leading to its proteasomal degradation.
Runager K et al. Targeting O-glycosyltransferase (OGT) to promote healing of diabetic skin wounds. J Biol Chem289:5462-6 (2014).
Read more (PubMed: 24398691) »
Rogacka D et al. Expression of GFAT1 and OGT in podocytes: transport of glucosamine and the implications for glucose uptake into these cells. J Cell Physiol225:577-84 (2010).
Read more (PubMed: 20506529) »