使用敲除细胞株进行验证
重组
RabMAb

Anti-Ogg1抗体[EPR4664(2)] (ab124741)

概述

  • 产品名称
    Anti-Ogg1抗体[EPR4664(2)]
    参阅全部 Ogg1 一抗
  • 描述
    兔单克隆抗体[EPR4664(2)] to Ogg1
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Ogg1 aa 300 to the C-terminus (C terminal).

  • 阳性对照
    • WB: HeLa, JAR, Raji, NCCIT and HepG2 cell lysates. Human cerebellum tissue lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能
      DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
    • 组织特异性
      Ubiquitous.
    • 疾病相关
      Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
    • 序列相似性
      Belongs to the type-1 OGG1 family.
    • 细胞定位
      Mitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 8 hydroxyguanine DNA glycosylase antibody
      • 8 oxoguanine DNA glycosylase 1 antibody
      • 8 oxoguanine DNA glycosylase antibody
      • AP lyase antibody
      • DNA (apurinic or apyrimidinic site) lyase antibody
      • DNA apurinic or apyrimidinic site lyase antibody
      • DNA lyase antibody
      • DNA-(apurinic or apyrimidinic site) lyase antibody
      • HMMH antibody
      • HOGG 1 antibody
      • HOGG1 antibody
      • MMH antibody
      • MUTM antibody
      • N glycosylase antibody
      • Ogg 1 antibody
      • Ogg1 antibody
      • OGG1_HUMAN antibody
      • OGH 1 antibody
      • OGH1 antibody
      see all

    图片



    • Predicted band size : 39 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Ogg1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab124741 observed at 38 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab124741 was shown to recognize Ogg1 when Ogg1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ogg1 knockout samples were subjected to SDS-PAGE. ab124741 and ab18058 (loading control to vinculin) were diluted 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Ogg1 antibody [EPR4664(2)] (ab124741) at 1/50000 dilution (purified)

      Lane 1 : JAR cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Ogg1 antibody [EPR4664(2)] (ab124741) at 1/10000 dilution (unpurified)

      Lane 1 : Human cerebellum tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    实验方案

    文献

    This product has been referenced in:
    • Yang C  et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res 45:739-748 (2017). Read more (PubMed: 27794043) »
    • Sassa A  et al. Xeroderma Pigmentosum Group A Suppresses Mutagenesis Caused by Clustered Oxidative DNA Adducts in the Human Genome. PLoS One 10:e0142218 (2015). WB ; Human . Read more (PubMed: 26559182) »

    See all 8 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular Smooth Mucle Cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Vascular Smooth Mucle Cell
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 May 16 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (various tumor cells types)
    Loading amount
    200000 cells
    Specification
    various tumor cells types
    Gel Running Conditions
    Reduced Denaturing (10-20% Tris Glycine)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 May 02 2013

    The exact sequence of the immunogen used to raise this antibody (ab124741) is commercially sensitive information and unfortunately, I am unable to release it.
    However, I can confirm that the sequence corresponds to the isoform 1A of human OGG1.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册