Anti-Ogg1抗体(ab62826)

概述

  • 产品名称Anti-Ogg1抗体
    参阅全部 Ogg1 一抗
  • 描述
    山羊多克隆抗体to Ogg1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide:

    C-HPKTSQAKGPSPLANK

    , corresponding to internal sequence amino acids 282-297 of Rat Ogg1

  • 阳性对照
    • Rat Spleen lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).
ELISA 1/128000.

靶标

  • 功能DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
  • 序列相似性Belongs to the type-1 OGG1 family.
  • 细胞定位Mitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 8 hydroxyguanine DNA glycosylase antibody
    • 8 oxoguanine DNA glycosylase 1 antibody
    • 8 oxoguanine DNA glycosylase antibody
    • AP lyase antibody
    • DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA apurinic or apyrimidinic site lyase antibody
    • DNA lyase antibody
    • DNA-(apurinic or apyrimidinic site) lyase antibody
    • HMMH antibody
    • HOGG 1 antibody
    • HOGG1 antibody
    • MMH antibody
    • MUTM antibody
    • N glycosylase antibody
    • Ogg 1 antibody
    • Ogg1 antibody
    • OGG1_HUMAN antibody
    • OGH 1 antibody
    • OGH1 antibody
    see all

Anti-Ogg1 antibody 图像

Anti-Ogg1 antibody (ab62826)参考文献

ab62826 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Rat Cell lysate - nuclear (rat aortic smooth muscle cells)
Specification rat aortic smooth muscle cells
Treatment transfected
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

提交于 Jun 24 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (mouse embryonic fibroblast cells)
Loading amount 35 µg
Specification mouse embryonic fibroblast cells
Treatment 0, 1, 10, 100, 1000 uM BSO for 24 hours
Gel Running Conditions Reduced Denaturing (12.5% gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - nuclear (MH1C1 cell (Rat hepatoma))
Loading amount 70 µg
Specification MH1C1 cell (Rat hepatoma)
Gel Running Conditions Reduced Denaturing (12.5% gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Nov 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"