概述

  • 产品名称
    Anti-OGDH抗体- C-terminal
    参阅全部 OGDH 一抗
  • 描述
    兔多克隆抗体to OGDH - C-terminal
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within C terminal amino acids 705-1000 of Human OGDH (Uniprot ID: Q02218).

  • 阳性对照
    • 293T, A431, H1299, HeLa S3, HepG2, MOLT4, Mouse brain and Raji whole cell lysates; HeLa cells; Human Cal27 xenograft tissue.

性能

应用

Our Abpromise guarantee covers the use of ab137773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

靶标

  • 功能
    The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 序列相似性
    Belongs to the alpha-ketoglutarate dehydrogenase family.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2 oxoglutarate dehydrogenase antibody
    • 2 oxoglutarate dehydrogenase complex component E1 antibody
    • 2 oxoglutarate dehydrogenase mitochondrial antibody
    • 2-oxoglutarate dehydrogenase antibody
    • 2-oxoglutarate dehydrogenase complex component E1 antibody
    • AKGDH antibody
    • Alpha ketoglutarate dehydrogenase antibody
    • Alpha-ketoglutarate dehydrogenase antibody
    • E1k antibody
    • mitochondrial antibody
    • ODO1_HUMAN antibody
    • OGDC antibody
    • OGDC E1 antibody
    • OGDC-E1 antibody
    • OGDH antibody
    • Oxoglutarate (alpha ketoglutarate) dehydrogenase (lipoamide) antibody
    • Oxoglutarate decarboxylase antibody
    • Oxoglutarate dehydrogenase (lipoamide) antibody
    • Oxoglutarate dehydrogenase (succinyl transferring) antibody
    see all

图片

  • All lanes : Anti-OGDH antibody - C-terminal (ab137773) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : HepG2 whole cell lysate
    Lane 6 : MOLT4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 116 kDa
  • Anti-OGDH antibody - C-terminal (ab137773) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 116 kDa
  • Immunofluorescence analysis of methanol-fixed HeLa cells labelling ODGH using ab137773 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labelling OGDH with ab135660 at 1/500 dilution

文献

This product has been referenced in:
  • Mailloux RJ  et al. Induction of mitochondrial reactive oxygen species production by GSH mediated S-glutathionylation of 2-oxoglutarate dehydrogenase. Redox Biol 8:285-97 (2016). WB ; Mouse . Read more (PubMed: 26928132) »
  • Gao J  et al. CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins. J Cell Biol 207:213-23 (2014). Read more (PubMed: 25349259) »

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Rat Tissue lysate - whole (Retina, RPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina, RPE
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK, ARPE19)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
HEK, ARPE19
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 19 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, liver, retina, RPE)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Brain, liver, retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 19 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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