概述

  • 产品名称
  • 描述
    山羊多克隆抗体to ODZ3
  • 经测试应用
    适用于: IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Chicken, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    C-NNPASKIHDVKDY

    , corresponding to internal sequence amino acids 2390-2402 of Human ODZ3 (NP_001073946.1).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab82575 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
IHC-P
ICC/IF
  • 应用说明
    ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 1.5 - 3 µg/ml.

    Peptide ELISA: Antibody detection limit dilution 1/16000.

    WB: Preliminary experiments in Human brain lysates and in lysates of cell line NIH 3T3 gave no specific signal but low background (at antibody concentration up to 1µg/ml).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Involved in neural development, regulating the establishment of proper connectivity within the nervous system. Promotes axon guidance and homophilic cell adhesion. Plays a role in the development of the visual pathway; regulates the formation in ipsilateral retinal mapping to both the dorsal lateral geniculate nucleus (dLGN) and the superior colliculus (SC). May be involved in the differentiation of the fibroblast-like cells in the superficial layer of mandibular condylar cartilage into chondrocytes. May function as a cellular signal transducer.
    • 组织特异性
      Expressed in adult and fetal brain, slightly lower levels in testis and ovary, and intermediate levels in all other peripheral tissues examined. Not expressed in spleen or liver. Expression was high in brain, with highest levels in amygdala and caudate nucleus, followed by thalamus and subthalamic nucleus.
    • 疾病相关
      Microphthalmia, isolated, with coloboma, 9
    • 序列相似性
      Belongs to the tenascin family. Teneurin subfamily.
      Contains 8 EGF-like domains.
      Contains 5 NHL repeats.
      Contains 1 teneurin N-terminal domain.
      Contains 23 YD repeats.
    • 结构域
      EGF-like domains 2 and 5 which have an odd number of cysteines might enable the formation of intermolecular disulfide bonds.
      Cytoplasmic proline-rich regions could serve as docking domains for intracellular SH3-containing proteins.
    • 细胞定位
      Membrane. Cell projection, axon.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ10474 antibody
      • FLJ10886 antibody
      • KIAA1455 antibody
      • odd Oz Ten m homolog 3 antibody
      • odz odd Oz ten m homolog 3 (Drosophila) antibody
      • odz odd Oz ten m homolog 3 antibody
      • ODZ3 like protein antibody
      • Protein Odd Oz ten m homolog 3 antibody
      • Protein Odd Oz/ten-m homolog 3 antibody
      • Ten 3 antibody
      • Ten M3 antibody
      • Ten-3 antibody
      • Ten-m3 antibody
      • TEN3_HUMAN antibody
      • Tenascin M3 antibody
      • Tenascin-M3 antibody
      • Teneurin 3 antibody
      • Teneurin transmembrane protein 3 antibody
      • Teneurin-3 antibody
      • Teneurin3 antibody
      • tenm3 antibody
      • TNM3 antibody
      see all

    图片

    • ab82575 staining ODZ in human cerebellum tissue by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Primary antibody used at 1.5µg/ml.
      Note the nuclear staining of the granular cell layer.
    • ab82575 staining ODZ3 in HeLa cells by Immunocytochemistry/ Immunofluorescence. Primary antibody used at 10µg/ml.

    文献

    ab82575 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Sample
    Chicken Tissue sections (chicken hindbrain)
    Specification
    chicken hindbrain
    Permeabilization
    Yes - 0.1% PBS-TritonX
    Fixative
    Paraformaldehyde
    Username

    Karli Montague

    Verified customer

    提交于 Mar 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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