Anti-Oct4抗体[PR-2H9] (ab95854)


  • 产品名称Anti-Oct4抗体[PR-2H9]
    参阅全部 Oct4 一抗
  • 描述
    小鼠单克隆抗体[PR-2H9] to Oct4
  • 经测试应用适用于: WB, Indirect ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human Oct4 aa 1-212.

  • 阳性对照
    • Recombinant Oct4 protein, HEK293-Oct-4 transfectant cell lysate, NTERA-2 cell lysate, and 2102 cell lysate.
  • 常规说明

    This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.



Our Abpromise guarantee covers the use of ab95854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 39 kDa.
Indirect ELISA 1/200 - 1/1000.


  • 功能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 组织特异性Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • 序列相似性Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 发展阶段Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • 结构域The POU-specific domain mediates interaction with PKM2.
  • 翻译后修饰Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • 细胞定位Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Octamer binding transcription factor 4 antibody
    • MGC22487 antibody
    • Oct 3 antibody
    • Oct 4 antibody
    • Oct-3 antibody
    • Oct-4 antibody
    • OCT3 antibody
    • Oct4 antibody
    • Octamer binding protein 3 antibody
    • Octamer binding protein 4 antibody
    • Octamer binding transcription factor 3 antibody
    • Octamer-binding protein 3 antibody
    • Octamer-binding protein 4 antibody
    • Octamer-binding transcription factor 3 antibody
    • OTF 3 antibody
    • OTF 4 antibody
    • OTF-3 antibody
    • OTF3 antibody
    • OTF4 antibody
    • PO5F1_HUMAN antibody
    • POU class 5 homeobox 1 antibody
    • POU domain class 5 transcription factor 1 antibody
    • POU domain transcription factor OCT4 antibody
    • POU domain, class 5, transcription factor 1 antibody
    • POU-type homeodomain-containing DNA-binding protein antibody
    • POU5F1 antibody
    see all

Anti-Oct4 antibody [PR-2H9] 图像

  • Indirect ELISA detection of immobilized Oct4 (full length) with titrated amounts of ab95854. No binding is seen to irrelevant recombinant proteins Nanog or SOX2.

  • Lane 1 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 0.1 µg/ml
    Lanes 2 - 6 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 1 µg/ml

    Lane 1 : Recombinant Oct-4 protein at 0.25 µg
    Lane 2 : Recombinant Oct-4 protein at 0.25 µg
    Lane 3 : Untransfected HEK293 lysate at 20 µg
    Lane 4 : HEK293-Oct-4 transfectant cell lysate at 20 µg
    Lane 5 : NTERA-2 cell lysate at 20 µg
    Lane 6 : 2102 cell lysate at 20 µg

    Predicted band size : 39 kDa

Anti-Oct4 antibody [PR-2H9] (ab95854)参考文献

ab95854 has not yet been referenced specifically in any publications.

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