This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 39 kDa.
1/200 - 1/1000.
功能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
组织特异性Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
序列相似性Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
发展阶段Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
结构域The POU-specific domain mediates interaction with PKM2.
翻译后修饰Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
细胞定位Nucleus. Expressed in a diffuse and slightly punctuate pattern.
Indirect ELISA detection of immobilized Oct4 (full length) with titrated amounts of ab95854. No binding is seen to irrelevant recombinant proteins Nanog or SOX2.
Western blot - Anti-Oct4 [PR-2H9] antibody (ab95854)
Lane 1 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 0.1 µg/ml Lanes 2 - 6 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 1 µg/ml
Lane 1 : Recombinant Oct-4 protein at 0.25 µg Lane 2 : Recombinant Oct-4 protein at 0.25 µg Lane 3 : Untransfected HEK293 lysate at 20 µg Lane 4 : HEK293-Oct-4 transfectant cell lysate at 20 µg Lane 5 : NTERA-2 cell lysate at 20 µg Lane 6 : 2102 cell lysate at 20 µg
Predicted band size : 39 kDa
Anti-Oct4 antibody [PR-2H9] (ab95854)参考文献
has not yet been referenced specifically in any publications.