概述

  • 产品名称Anti-Oct4抗体
    参阅全部 Oct4 一抗
  • 描述
    山羊多克隆抗体to Oct4
  • 经测试应用适用于: Flow Cyt, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Oct4 aa 300 to the C-terminus (C terminal).
    (Peptide available as ab20650)

  • 阳性对照
    • Human or Mouse Embryonic Stem Cells

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab27985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).

靶标

  • 功能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 组织特异性Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • 序列相似性Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 发展阶段Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • 结构域The POU-specific domain mediates interaction with PKM2.
  • 翻译后修饰Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • 细胞定位Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Octamer binding transcription factor 4 antibody
    • MGC22487 antibody
    • Oct 3 antibody
    • Oct 4 antibody
    • Oct-3 antibody
    • Oct-4 antibody
    • OCT3 antibody
    • Oct4 antibody
    • Octamer binding protein 3 antibody
    • Octamer binding protein 4 antibody
    • Octamer binding transcription factor 3 antibody
    • Octamer-binding protein 3 antibody
    • Octamer-binding protein 4 antibody
    • Octamer-binding transcription factor 3 antibody
    • OTF 3 antibody
    • OTF 4 antibody
    • OTF-3 antibody
    • OTF3 antibody
    • OTF4 antibody
    • PO5F1_HUMAN antibody
    • POU class 5 homeobox 1 antibody
    • POU domain class 5 transcription factor 1 antibody
    • POU domain transcription factor OCT4 antibody
    • POU domain, class 5, transcription factor 1 antibody
    • POU-type homeodomain-containing DNA-binding protein antibody
    • POU5F1 antibody
    see all

Anti-Oct4 antibody 图像

  • ab27985 recognizes Oct4 in human embryonic stem cells. The upper panel shows DAPI staining while the lower panel shows ab27985 staining.

    See Abreview

  • ab27985 recognizes Oct4 in mouse embryonic stem cells. DAPI staining is shown in the top panel while ab27985 staining is shown in the lower panel.

    See Abreview

  • All lanes : Anti-Oct4 antibody (ab27985) at 1 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat IgG secondary antibody (ab28445) at 1/10000 dilution

    Predicted band size : 39 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    The antibody recognised a band corresponding to Oct4 in mouse and human embryonic stem cell lysates.

  • ab27985 staining Oct4 in human embryonic carcinoma cells by Flow Cytometery. Cells were fixed in paraformaldehyde and permeabilized in saponin buffer. The primary antibody was diluted 1:500 (PBS + 10% BSA) and incubated with sample for 1 hour at 4°C. A FITC conjugated Donkey polyclonal to  goat IgG, diluted 1:200 was used as secondary. The red histogram is secondary only control whilst green and blue repersents Oct4 expression in untreated and partially differentiated with 10nM Trans-Retinoic acid for 14 days. 

    See Abreview

Anti-Oct4 antibody (ab27985)参考文献

This product has been referenced in:
  • Caiazzo M  et al. Defined three-dimensional microenvironments boost induction of pluripotency. Nat Mater N/A:N/A (2016). IF ; Mouse . Read more (PubMed: 26752655) »
  • Peng X  et al. Germline transmission of an embryonic stem cell line derived from BALB/c cataract mice. PLoS One 9:e90707 (2014). Flow Cyt, ICC/IF ; Mouse . Read more (PubMed: 24595217) »

See all 20 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cell (Embryonic Stem Cell)
Permeabilization Yes - 0.2% Triton X
Specification Embryonic Stem Cell
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Aug 01 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample Mouse Tissue sections (mice embryo 14.5 dpc)
Specification mice embryo 14.5 dpc
Permeabilization Yes - Tween-20
Fixative Formaldehyde
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提交于 Mar 10 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample Dog Tissue sections (MDCK pellets in paraffin)
Specification MDCK pellets in paraffin
Permeabilization Yes - Tween-20
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Mar 04 2015

Thank you for the details provided. I am sorry you are having difficulty with these products.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab27985 and ab96916.

1) Increase the Tri...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic stem cell)
Loading amount 1e+006 cells
Specification embryonic stem cell
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Abcam user community

Verified customer

提交于 Aug 14 2012

Thank you for contacting Abcam.

Based on the information we have for this antibody, it may be that it is not suitable for IHC-Fr on sections. However based on the information you have provided, you could try to increase the primary antibody c...

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Thank you for your call today and for letting us know about the trouble with these antibodies.

Please keep me updated about the results with these antibodies after trying the alterations that we discussed. Here is the article that I mentioned...

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Thank you for contacting us. The closest match we have to ab28445 is ab96930. This is an anti-goat IgG, raised in rabbit, conjugated to DyLight® 650, which has an excitation maximum at 654nm and an emission maximum at 673nm. The Alexa Fluor 680 fluo...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E12.5 testis)
Specification E12.5 testis
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton or 1% Triton
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 25 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 08 2011

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