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Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 38 kDa).
功能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
组织特异性Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
序列相似性Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
发展阶段Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
结构域The POU-specific domain mediates interaction with PKM2.
翻译后修饰Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
细胞定位Nucleus. Expressed in a diffuse and slightly punctuate pattern.
The predicted molecular weight of Oct4 is 38 kDa (SwissProt), however we expect to observe a banding pattern around 44 kDa.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125949 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Anti-Oct4 antibody (ab125949)参考文献
has not yet been referenced specifically in any publications.