RabMAb

Anti-Oct-2抗体[EPR542] (ab92458)

概述

  • 产品名称Anti-Oct-2抗体[EPR542]
    参阅全部 Oct-2 一抗
  • 描述
    兔单克隆抗体[EPR542] to Oct-2
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Oct-2 aa 150-250.
    Database link: P09086

  • 阳性对照
    • Daudi cell lysate and Human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Oct-2 antibody (HRP) [EPR542] (ab200602)

性能

应用

Our Abpromise guarantee covers the use of ab92458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC
Flow Cyt
  • 应用说明Flow Cyt: 1/50.
    ICC: 1/250 - 1/500.
    IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
    WB: 1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
    • 组织特异性Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
    • 序列相似性Belongs to the POU transcription factor family. Class-2 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • class 2 antibody
      • Lymphoid restricted immunoglobulin octamer binding protein antibody
      • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
      • NF A2 antibody
      • Oct 2 antibody
      • Oct-2 antibody
      • Octamer binding transcription factor 2 antibody
      • Octamer-binding protein 2 antibody
      • Octamer-binding transcription factor 2 antibody
      • OTF-2 antibody
      • OTF2 antibody
      • PO2F2_HUMAN antibody
      • POU domain antibody
      • POU domain class 2 transcription factor 2 antibody
      • POU2F2 antibody
      • transcription factor 2 antibody
      see all

    Anti-Oct-2 antibody [EPR542] 图像

    • ab92458, at 1:250 staining Oct-2 in Human tonsil paraffin embeeded section by immunohistochemistry.

    • Anti-Oct-2 antibody [EPR542] (ab92458) at 1/1000 dilution + Daudi lysate at 10 µg

      Secondary
      HRP conjugated goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing Raji cells stained with ab92458 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92458, 1:50) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Oct-2 antibody [EPR542] (ab92458)参考文献

    ab92458 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry.

    I can confirm that this antibody (ab92458) has been tested for these applications (Flow, ICC and WB).

    If you need any further assistance in the future, please do not hesitate to contact me.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"