概述

  • 产品名称Anti-Oct-2抗体
    参阅全部 Oct-2 一抗
  • 描述
    兔多克隆抗体to Oct-2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Oct-2 aa 1-50 (N terminal).
    Sequence:

    MVHSSMGAPEIRMSKPLEAEKQGLDSPSEHTDTERNGPDTNHQNPQNKTS


    Database link: P09086

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab49247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

靶标

  • 功能Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
  • 组织特异性Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
  • 序列相似性Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • class 2 antibody
    • Lymphoid restricted immunoglobulin octamer binding protein antibody
    • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
    • NF A2 antibody
    • Oct 2 antibody
    • Oct-2 antibody
    • Octamer binding transcription factor 2 antibody
    • Octamer-binding protein 2 antibody
    • Octamer-binding transcription factor 2 antibody
    • OTF-2 antibody
    • OTF2 antibody
    • PO2F2_HUMAN antibody
    • POU domain antibody
    • POU domain class 2 transcription factor 2 antibody
    • POU2F2 antibody
    • transcription factor 2 antibody
    see all

Anti-Oct-2 antibody 图像

  • Anti-Oct-2 antibody (ab49247) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-Oct-2 antibody (ab49247)参考文献

ab49247 has not yet been referenced specifically in any publications.

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