Anti-Oct-1抗体[POU5I097] (ab51363)
Key features and details
- Mouse monoclonal [POU5I097] to Oct-1
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-Oct-1抗体[POU5I097]
参阅全部 Oct-1 一抗 -
描述
小鼠单克隆抗体[POU5I097] to Oct-1 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Oct-1.
Database link: P14859 -
阳性对照
- HeLa cells, HEK293 whole cell lysate
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
-
纯度
Protein G purified -
纯化说明
Filtered through a 0.22 µm membrane. -
克隆
单克隆 -
克隆编号
POU5I097 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51363于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
1/50. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
|
|
ICC/IF | (1) |
1/10.
|
说明 |
---|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/50. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). |
ICC/IF
1/10. |
靶标
-
功能
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes. -
组织特异性
Ubiquitous. Isoform 2 is lymphocyte-specific. -
序列相似性
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
翻译后修饰
Phosphorylated by PRKDC. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 5451 Human
- Omim: 164175 Human
- SwissProt: P14859 Human
- Unigene: 283402 Human
-
别名
- FLJ42836 antibody
- NF A1 antibody
- NF-A1 antibody
see all
图片
-
Ab51363 at 1:10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1:200 dilution.
-
Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa -
Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
-
Datasheet download
文献 (3)
ab51363 被引用在 3 文献中.
- He W et al. DNA methylation integratedly modulates the expression of Pit-Oct-Unt transcription factors in esophageal squamous cell carcinoma. J Cancer 12:1634-1643 (2021). PubMed: 33613750
- Kang Z et al. Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors. J Mol Biol 389:595-605 (2009). PubMed: 19389405
- Thum T & Borlak J Lox-1 receptor blockade abrogates OXLDL induced oxidative DNA damage and prevents activation of the transcriptional repressor OCT-1 in human coronary arterial endothelium. J Biol Chem : (2008). ICC/IF ; Human . PubMed: 18390905