- 数据表
- 具体的参考文献 (55)
- 实验方案
概述
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产品名称Anti-Occludin抗体
参阅全部 Occludin 一抗 -
描述兔多克隆抗体to Occludin
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特异性The immunogen used to raise this antibody has 78% homology with the Mouse Occludin protein and the Rat Occludin protein. Some customers have successfully used ab31721 with Mouse/Rat lysates/tissue, however we have not been successful detecting Occludin in these species in our own testing. Please contact Abcam Scientific Support for more information.
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经测试应用适用于: IHC-FoFr, In-Cell ELISA, ICC/IF, IHC-Pmore details
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种属反应性与反应: Rat, Human, Pig
预测可用于: Dog
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Occludin.
(Peptide available as ab34440.)
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阳性对照
- IHC-P: Normal human kidney tissue. ICC/IF: MCF7 cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab31721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20978075 | |
| In-Cell ELISA | Use at an assay dependent concentration. PubMed: 22689949 | |
| ICC/IF | Use a concentration of 1 - 2 µg/ml. | |
| IHC-P | 1/100. PubMed: 19828778 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
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组织特异性Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
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疾病相关Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
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序列相似性Belongs to the ELL/occludin family.
Contains 1 MARVEL domain. -
结构域The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
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翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
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细胞定位Membrane. Cell junction > tight junction.
- Information by UniProt
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数据库链接
- Entrez Gene: 403844 Dog
- Entrez Gene: 100506658 Human
- Entrez Gene: 397236 Pig
- Entrez Gene: 83497 Rat
- Omim: 602876 Human
- SwissProt: Q28269 Dog
- SwissProt: Q16625 Human
- SwissProt: Q6P6T5 Rat
see all -
别名
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
see all
Anti-Occludin antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody (ab31721)IHC image of Occludin staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31721, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody (ab31721)Image is courtesy of an anonymous AbReviewab31721 staining Occludin in Human Breast cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1) and blocked with 0.1% milk for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% milk) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit polyclonal (1/1500) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody (ab31721)ICC/IF image of ab31721 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31721, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Occludin antibody (ab31721)参考文献
This product has been referenced in:
- Liu HJ et al. The flavonoid TL-2-8 induces cell death and immature mitophagy in breast cancer cells via abrogating the function of the AHA1/Hsp90 complex. Acta Pharmacol Sin N/A:N/A (2017). WB ; Human . Read more (PubMed: 28504248) »
- Chelakkot C et al. Intestinal Epithelial Cell-Specific Deletion of PLD2 Alleviates DSS-Induced Colitis by Regulating Occludin. Sci Rep 7:1573 (2017). WB, IHC-P, ICC/IF ; Mouse . Read more (PubMed: 28484281) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"