存放说明Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解度概述Soluble in water
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
ab7963 staining c-Fos in NIH 3T3 cells treated with obestatin (ab120071), by ICC/IF. Increase in c-Fos expression correlates with increased concentration of obestatin, as described in literature. The cells were incubated at 37°C for 3h in media containing different concentrations of ab120071 (obestatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7963 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
Pan W et al. Differential BBB interactions of three ingestive peptides: obestatin, ghrelin, and adiponectin. Peptides27:911-6 (2006).
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