概述

  • 产品名称Anti-OAS1抗体
    参阅全部 OAS1 一抗
  • 描述
    兔多克隆抗体to OAS1
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from within a region of C terminal amino acids 323-372 (EAWLNYPCFK NWDGSPVSSW ILLAESNSAD DETDDPRRYQ KYGYIGTHEY) of Human OAS1 (NP_058132)

  • 阳性对照
    • 293T cell lysate IF/ICC: HepG2 cell line

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab82666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.Can be blocked with OAS1 peptide (ab196700). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能May play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis.
  • 序列相似性Belongs to the 2-5A synthase family.
  • 细胞定位Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • (2 5')oligo(A) synthetase 1 antibody
    • (2-5'')oligo(A) synthase 1 antibody
    • 2 5 Oligoadenylate Synthetase 1 antibody
    • 2 5' oligo A synthase 1 antibody
    • 2 5' oligo A synthetase 1 antibody
    • 2 5A synthase 1 antibody
    • 2 5A synthetase 1 antibody
    • 2' 5' oligo A synthetase 1 antibody
    • 2' 5' oligoadenylate synthetase 1 40/46kDa antibody
    • 2' 5' oligoadenylate synthetase 1 antibody
    • 2' 5' oligoisoadenylate synthetase 1 antibody
    • 2''-5''-oligoadenylate synthase 1 antibody
    • 2'5' oligo A synthetase 1 antibody
    • 2'5' oligoadenylate synthetase 1 antibody
    • 2'5' oligoisoadenylate synthetase 1 antibody
    • 2-5A synthase 1 antibody
    • E18/E16 antibody
    • IFI 4 antibody
    • IFI4 antibody
    • OAS 1 antibody
    • OAS1 antibody
    • OAS1_HUMAN antibody
    • OIAS antibody
    • OIASI antibody
    • p46/p42 OAS antibody
    see all

Anti-OAS1 antibody 图像

  • ICC/IF image of ab82666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-OAS1 antibody (ab82666) at 1 µg/ml (5% skim milk/ PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 32 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry of Tonsil tissue at an antibody concentration of 5µg/ml using anti-OAS1 antibody (ab82666)

Anti-OAS1 antibody (ab82666)参考文献

This product has been referenced in:
  • Li Q & Tainsky MA Higher miRNA tolerance in immortal Li-Fraumeni fibroblasts with abrogated interferon signaling pathway. Cancer Res 71:255-65 (2011). WB ; Human . Read more (PubMed: 21199806) »

See 1 Publication for this product

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