ICC/IF image of ab82666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - OAS1 antibody (ab82666)
Anti-OAS1 antibody (ab82666) at 1 µg/ml (5% skim milk/ PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 12%
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 46 kDa Observed band size : 46 kDa Additional bands at : 32 kDa,35 kDa. We are unsure as to the identity of these extra bands.