概述

  • 产品名称Anti-OAS1抗体
    参阅全部 OAS1 一抗
  • 描述
    小鼠单克隆抗体to OAS1
  • 经测试应用适用于: IHC-P, ICC/IF, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: SVSRRDKSKQ VWEAVLLPLS LLSMMDLRNT PAKSLDKFIE DYLLPDTCFR MQINHAIDII CGFLKERCFR GSSYPVCVSK VVKGGSSGKG TTLRGRSDAD , corresponding to amino acids 1-100 of Human OAS1

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能May play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis.
  • 序列相似性Belongs to the 2-5A synthase family.
  • 细胞定位Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • (2 5')oligo(A) synthetase 1 antibody
    • (2-5'')oligo(A) synthase 1 antibody
    • 2 5 Oligoadenylate Synthetase 1 antibody
    • 2 5' oligo A synthase 1 antibody
    • 2 5' oligo A synthetase 1 antibody
    • 2 5A synthase 1 antibody
    • 2 5A synthetase 1 antibody
    • 2' 5' oligo A synthetase 1 antibody
    • 2' 5' oligoadenylate synthetase 1 40/46kDa antibody
    • 2' 5' oligoadenylate synthetase 1 antibody
    • 2' 5' oligoisoadenylate synthetase 1 antibody
    • 2''-5''-oligoadenylate synthase 1 antibody
    • 2'5' oligo A synthetase 1 antibody
    • 2'5' oligoadenylate synthetase 1 antibody
    • 2'5' oligoisoadenylate synthetase 1 antibody
    • 2-5A synthase 1 antibody
    • E18/E16 antibody
    • IFI 4 antibody
    • IFI4 antibody
    • OAS 1 antibody
    • OAS1 antibody
    • OAS1_HUMAN antibody
    • OIAS antibody
    • OIASI antibody
    • p46/p42 OAS antibody
    see all

Anti-OAS1 antibody 图像

  • OAS1 antibody (ab55771) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
  • All lanes : Anti-OAS1 antibody (ab55771) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab55771 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55771, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab55771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55771, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-OAS1 antibody (ab55771)参考文献

ab55771 has not yet been referenced specifically in any publications.

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