ab74999 detects endogenous levels of total NXPH4 protein.
A synthesized peptide derived from an internal region of human NXPH4.
IHC-P: human brain tissue.
WB: extracts from COLO cells and HUVEC cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 33 kDa.
1/50 - 1/100.
NXPH4 is a neurexophilin. Mammalian brains contain four different neurexophilin proteins. Neurexophilins form a family of related glycoproteins that are proteolytically processed after synthesis and bind to alpha neurexins. The structure and characteristics of neurexophilins indicate that they function as neuropeptides that may signal via alpha neurexins and possibly other receptors.
Neurexophilin 4 antibody
NXPH 4 antibody
Western blot - Anti-NXPH4 antibody (ab74999)
All lanes : Anti-NXPH4 antibody (ab74999) at 1/500 dilution Lane 1 : Extracts from COLO cells Lane 2 : Extracts from HUVEC cells Lane 3 : Extracts from COLO cells plus 5µg immunizing peptide Lysates/proteins at 5 µg per lane. Predicted band size: 33 kDa Observed band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXPH4 antibody (ab74999)
Immunohistochemical analysis of NXPH4 in paraffin embedded human brain tissue using ab74999 at a 1/50 dilution.
Left image: untreated. Right image: treated with immunizing peptide.
has not yet been referenced specifically in any publications.
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