概述

  • 产品名称
  • 描述
    兔多克隆抗体to NXF3
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to a region of Human NXF3.

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab76430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

靶标

  • 功能
    May function as a tissue-specific nuclear mRNA export factor.
  • 组织特异性
    Expressed at high level in testis and at low level in a small number of tissues.
  • 序列相似性
    Belongs to the NXF family.
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 结构域
    Lacks C-terminal domain that mediates direct interactions with nucleoporins.
    Contains a novel CRM1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain.
    The RNA-binding domain is a non-canonical RNP-type domain.
  • 细胞定位
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Nuclear RNA export factor 3 antibody
    • NXF3 antibody
    • NXF3_HUMAN antibody
    • OTTHUMP00000023726 antibody
    • OTTHUMP00000023727 antibody
    • TAP like protein 3 antibody
    • TAP-like protein 3 antibody
    • TAPL 3 antibody
    • TAPL-3 antibody
    • TAPL3 antibody
    see all

图片



  • Predicted band size : 60 kDa
    Western blot analysis of HeLa cystosolic (lane 1) and nuclear (lane 2) lysates (7µg/lane) labeling NXF3 with ab76430 at 1:300. A HRP-conjugated anti-rabbit (1:500) was used as the secondary antibody.
  • Anti-NXF3 antibody (ab76430) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%

实验方案

文献

ab76430 has not yet been referenced specifically in any publications.

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