概述

  • 产品名称Anti-NXF1抗体
    参阅全部 NXF1 一抗
  • 描述
    兔多克隆抗体to NXF1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 449-598 of Human NXF1

  • 阳性对照
    • 293T, A431, HeLaS3, HepG2, Molt-4, Raji and HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab96078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
  • 组织特异性Expressed ubiquitously.
  • 序列相似性Belongs to the NXF family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 TAP-C domain.
  • 结构域The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
    The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
    The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
    The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
    The leucine-rich repeats are essential for the export of mRNA from the nucleus.
    The RNA-binding domain is a non-canonical RNP-type domain.
  • 细胞定位Nucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp667O0311 antibody
    • DmNXF1 antibody
    • MEX67 antibody
    • MEX67, yeast, homolog of antibody
    • Mex67p antibody
    • mRNA export factor TAP antibody
    • Mvb1 antibody
    • Nuclear RNA export factor 1 antibody
    • Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody
    • nxf 1 antibody
    • NXF1 antibody
    • NXF1_HUMAN antibody
    • Protein small bristles antibody
    • Sbr antibody
    • TAP antibody
    • Tip associating protein antibody
    • Tip-associated protein antibody
    • Tip-associating protein antibody
    see all

Anti-NXF1 antibody 图像

  • Anti-NXF1 antibody (ab96078) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 70 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96078 at 1/200 dilution.

Anti-NXF1 antibody (ab96078)参考文献

ab96078 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 40000 cells
Gel Running Conditions Reduced Denaturing (4-12% gradient gel)
Sample Zebrafish Tissue lysate - whole (10 embryos, animal cap)
Specification 10 embryos, animal cap
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Maximilian Krause

Verified customer

提交于 Jan 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"