概述

  • 产品名称Anti-NUSAP1抗体
    参阅全部 NUSAP1 一抗
  • 描述
    兔多克隆抗体to NUSAP1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide from a region within amino acids 125 - 175 of Human NUSAP1 (NP_057443.2)

  • 阳性对照
    • HeLa cell lysate 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab93779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 5-15µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 49 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
    • 序列相似性Belongs to the NUSAP family.
    • 结构域The KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.
    • 细胞定位Cytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2610201A12Rik antibody
      • AI481307 antibody
      • ANKT antibody
      • AW547774 antibody
      • BB165529 antibody
      • BM037 antibody
      • FLJ13421 antibody
      • LNP antibody
      • Nucleolar and spindle associated protein 1 antibody
      • Nucleolar and spindle-associated protein 1 antibody
      • Nucleolar protein ANKT antibody
      • NuSAP antibody
      • NUSAP_HUMAN antibody
      • nusap1 antibody
      • PRO0310p1 antibody
      • Q0310 antibody
      • RP23-22A15.4 antibody
      • SAPL antibody
      see all

    Anti-NUSAP1 antibody 图像

    • All lanes : Anti-NUSAP1 antibody (ab93779) at 0.4 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 49 kDa


      Exposure time : 3 minutes
    • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane
      All lanes : NUSAP1 antibody (ab93779) at 1 µg/ml
      Lane 1 : Lysate immunoprecipitated with ab93779
      Lane 2 : Lysate immunoprecipitated with a control IgG

      Developed using the ECL technique
      Exposure time : 30 seconds

    Anti-NUSAP1 antibody (ab93779)参考文献

    This product has been referenced in:
    • Xie P  et al. ATM-mediated NuSAP phosphorylation induces mitotic arrest. Biochem Biophys Res Commun 404:413-8 (2011). WB, IP ; Human . Read more (PubMed: 21130744) »

    See 1 Publication for this product

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