概述

  • 产品名称
  • 描述
    兔多克隆抗体to NUSAP1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide from a region within amino acids 125 - 175 of Human NUSAP1 (NP_057443.2)

  • 阳性对照
    • HeLa cell lysate 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab93779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 5-15µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 49 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
    • 序列相似性
      Belongs to the NUSAP family.
    • 结构域
      The KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.
    • 翻译后修饰
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.
    • 细胞定位
      Cytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2610201A12Rik antibody
      • AI481307 antibody
      • ANKT antibody
      • AW547774 antibody
      • BB165529 antibody
      • BM037 antibody
      • FLJ13421 antibody
      • LNP antibody
      • Nucleolar and spindle associated protein 1 antibody
      • Nucleolar and spindle-associated protein 1 antibody
      • Nucleolar protein ANKT antibody
      • NuSAP antibody
      • NUSAP_HUMAN antibody
      • nusap1 antibody
      • PRO0310p1 antibody
      • Q0310 antibody
      • RP23-22A15.4 antibody
      • SAPL antibody
      see all

    图片

    • All lanes : Anti-NUSAP1 antibody (ab93779) at 0.4 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 49 kDa


      Exposure time : 3 minutes
    • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane
      All lanes : NUSAP1 antibody (ab93779) at 1 µg/ml
      Lane 1 : Lysate immunoprecipitated with ab93779
      Lane 2 : Lysate immunoprecipitated with a control IgG

      Developed using the ECL technique
      Exposure time : 30 seconds

    文献

    This product has been referenced in:
    • Li C  et al. NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force. Nat Commun 7:10597 (2016). IF . Read more (PubMed: 26839278) »
    • Xie P  et al. ATM-mediated NuSAP phosphorylation induces mitotic arrest. Biochem Biophys Res Commun 404:413-8 (2011). WB, IP ; Human . Read more (PubMed: 21130744) »

    See all 2 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Different human cell lines)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Nupage)
    Loading amount
    40 µg
    Specification
    Different human cell lines
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    提交于 Jan 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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