The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 5-15µg/mg of lysate.
WB: 1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
Belongs to the NUSAP family.
The KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.
Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.
Cytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane All lanes : NUSAP1 antibody (ab93779) at 1 µg/ml Lane 1 : Lysate immunoprecipitated with ab93779 Lane 2 : Lysate immunoprecipitated with a control IgG
Developed using the ECL technique Exposure time : 30 seconds