Anti-Nurr1抗体[N1404] - ChIP Grade (ab41917)

概述

  • 产品名称Anti-Nurr1抗体[N1404] - ChIP Grade
    参阅全部 Nurr1 一抗
  • 描述
    小鼠单克隆抗体[N1404] to Nurr1 - ChIP Grade
  • 特异性Antibody does not recognize alpha and gamma forms.
  • 经测试应用适用于: WB, ChIP, ELISA, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment: CVQAQYGSSP QGASPASQSY SYHSSGEYSS DFLTPEFVKF SMDLTNTEIT ATTSLPSFST FMDNYSTGYD VKPPCLYQMP LSGQQSSIKV EDIQMHN, corresponding to amino acids 2-99 of Human Nurr1

  • 阳性对照
    • Rat cerebral nerve cell.

性能

应用

Our Abpromise guarantee covers the use of ab41917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Non reducing WB 3 µg/ml.
ChIP 1/100.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22800564

靶标

  • 功能Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
  • 组织特异性Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 发展阶段Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HZF 3 antibody
    • HZF3 antibody
    • Immediate-early response protein NOT antibody
    • Intermediate early receptor protein antibody
    • NGFI B/nur77 beta type transcription factor homolog antibody
    • NOT antibody
    • Nr4a2 antibody
    • NR4A2_HUMAN antibody
    • nuclear receptor of T cells antibody
    • nuclear receptor related 1 antibody
    • Nuclear receptor subfamily 4 group A member 2 antibody
    • Nur related protein 1 homolog antibody
    • nur related protein-1, human homolog of antibody
    • Nurr 1 antibody
    • Orphan nuclear receptor NR4A2 antibody
    • Orphan nuclear receptor NURR1 antibody
    • RNR 1 antibody
    • RNR1 antibody
    • T cell nuclear receptor NOT antibody
    • T-cell nuclear receptor NOT antibody
    • TINUR antibody
    • Transcriptionally inducible nuclear receptor antibody
    • Transcriptionally inducible nuclear receptor related antibody
    • Transcriptionally inducible nuclear receptor related 1 antibody
    • Transcriptionally-inducible nuclear receptor antibody
    see all

Anti-Nurr1 antibody [N1404] - ChIP Grade 图像

  • ChIP analysis using ab41917 binding Nurr1 in Mouse microglia whole cell lysate. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (1/100 in ChIP dilution buffer - 0.01% SDS, 1.1% Triton X-100, 1.2mM EDTA, 16.7mM Tris-HCl (pH8.1), 167mM NaCl, proteanase inhibitor cocktail) for 16 hours at 4°C. Protein binding was detected using real-time PCR.
    Negative Control: BV2 Mouse microglia cells.

    Positive control: LPS treated BV2 Mouse microglia cells.

     

    See Abreview

  • ab41917 staining Nurr1 rat cerebral nerve cell paraffin section (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.

Anti-Nurr1 antibody [N1404] - ChIP Grade (ab41917)参考文献

This product has been referenced in:
  • Navarro D  et al. Late Maternal Hypothyroidism Alters the Expression of Camk4 in Neocortical Subplate Neurons: A Comparison with Nurr1 Labeling. Cereb Cortex N/A:N/A (2013). Read more (PubMed: 23680840) »
  • Lungu G  et al. MicroRNA profiling and the role of microRNA-132 in neurodegeneration using a rat model. Neurosci Lett 553:153-8 (2013). Read more (PubMed: 23973300) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - whole cell (mouse microglia cells)
Specification mouse microglia cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 09 2013

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided and have following further questions for better understanding of the problem;
- For how l...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (macrophage)
Loading amount 200 µg
Specification macrophage
Treatment 20uM compound X and IP perfomred
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Sep 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (RAW cell (mouse macrophage))
Total protein in input 200 µg
Specification RAW cell (mouse macrophage)
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

提交于 Sep 07 2012

DISCOUNT CODE: 100%ABR-0000
Expiration date: 06. November 2012

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