Anti-NUR77抗体(ab73982)

概述

  • 产品名称Anti-NUR77抗体
    参阅全部 NUR77 一抗
  • 描述
    兔多克隆抗体to NUR77
  • 特异性ab73982 detects endogenous levels of total NUR77.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human NUR77 around the phosphorylation site of serine 351 (L-P-SP-K-P).

  • 阳性对照
    • Human colon carcinoma tissue. Extracts from COS7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab73982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.
  • 组织特异性Fetal muscle and adult liver, brain and thyroid.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Early response protein NAK1 antibody
    • GFRP 1 antibody
    • GFRP antibody
    • GFRP1 antibody
    • Growth factor inducible nuclear protein N10 antibody
    • Growth Factor Inducible Nuclear Protein NP10 antibody
    • Growth Factor Response Protein 1 antibody
    • Hbr1 antibody
    • HMR antibody
    • Hormone Receptor antibody
    • MGC9485 antibody
    • N10 antibody
    • N10 nuclear protein antibody
    • NAK 1 antibody
    • NAK1 antibody
    • Nerve growth factor IB nuclear receptor variant 1 antibody
    • NGFIB antibody
    • NP 10 antibody
    • NP10 antibody
    • NR4A1 antibody
    • NR4A1_HUMAN antibody
    • Nuclear hormone receptor NUR/77 antibody
    • Nuclear Hormone Receptor TR3 antibody
    • Nuclear receptor subfamily 4 group A member 1 antibody
    • NUR77 antibody
    • NUR77, mouse, homolog of antibody
    • Orphan nuclear receptor HMR antibody
    • Orphan nuclear receptor NR4A1 antibody
    • Orphan nuclear receptor TR3 antibody
    • Orphan receptor tr3 antibody
    • Receptor NGFIB antibody
    • ST 59 antibody
    • ST-59 antibody
    • ST59 antibody
    • Steroid receptor TR3 antibody
    • Testicular receptor 3 antibody
    • TR 3 antibody
    • TR3 antibody
    • TR3 orphan receptor antibody
    see all

Anti-NUR77 antibody 图像

  • All lanes : Anti-NUR77 antibody (ab73982) at 1/500 dilution

    Lane 1 : Extracts from COS-7 cells
    Lane 2 : Extracts from COS-7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ab73982, at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-NUR77 antibody (ab73982)参考文献

ab73982 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (PC12 cells)
Loading amount 30 µg
Specification PC12 cells
Treatment 300nM dbcAMP for 1hr
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"