The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 105-8 cells. in a final volume of 100 µL
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.
Fetal muscle and adult liver, brain and thyroid.
Belongs to the nuclear hormone receptor family. NR4 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Flow cytometry images showing staining of unstimulated (left) and 2-hr PMA and Ionomycin stimulated (right) C57BL/6 thymocytes with an Anti-Mouse CD4 APC antibody, followed by intracellular staining with 0.5 µg of ab95659 using a Foxp3 Staining Buffer Set. Total viable cells were used for analysis.