重组
RabMAb

Anti-NUP98抗体[EPR6678] -核Pore Marker (ab124980)

概述

  • 产品名称
    Anti-NUP98抗体[EPR6678] -核Pore Marker
    参阅全部 NUP98 一抗
  • 描述
    兔单克隆抗体[EPR6678] to NUP98 -核Pore Marker
  • 特异性
    ab124980 recognises Nuclear pore complex protein Nup96 of Nuclear pore complex protein Nup98-Nup96.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NUP98 aa 1600-1700. The immunogen sequence is within Nuclear pore complex protein Nup96 of the Nuclear pore complex protein Nup98-Nup96.
    Database link: P52948

  • 阳性对照
    • HeLa, SH-SY5Y, and Caco-2 cell lysates and Human colon tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 188 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC/IF 1/50 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
    • 疾病相关
      Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
      Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
      Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
      Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
      Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
    • 序列相似性
      Belongs to the nucleoporin GLFG family.
      Contains 1 peptidase S59 domain.
    • 结构域
      Contains G-L-F-G repeats.
    • 翻译后修饰
      Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
    • 细胞定位
      Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 96 kDa nucleoporin antibody
      • 98 kDa nucleoporin antibody
      • ADAR2 antibody
      • ADIR2 antibody
      • GLFG-repeat containing nucleoporin antibody
      • Nuclear pore complex protein Nup96 antibody
      • Nuclear pore complex protein Nup98 Nup96 antibody
      • Nucleoporin 98kD antibody
      • nucleoporin 98kDa antibody
      • Nucleoporin Nup96 antibody
      • Nucleoporin Nup98 antibody
      • NUP196 antibody
      • NUP96 antibody
      • Nup98 antibody
      • Nup98-Nup96 antibody
      • NUP98_HUMAN antibody
      see all

    图片

    • All lanes : Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : Caco-2 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 188 kDa
    • ab124980, at 1/50 to 1/100, staining NUP98 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    文献

    This product has been referenced in:
    • Light WH  et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). Read more (PubMed: 23555195) »

    See 1 Publication for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (postnatal 60days brain)
    Permeabilization
    Yes - 0.4%triton 30min
    Specification
    postnatal 60days brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Dr. liang zhang

    Verified customer

    提交于 Feb 03 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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