重组
RabMAb

Anti-NUP98抗体[EPR6678] -核Pore Marker (ab124980)

概述

  • 产品名称
    Anti-NUP98抗体[EPR6678] -核Pore Marker
    参阅全部 NUP98 一抗
  • 描述
    兔单克隆抗体[EPR6678] to NUP98 -核Pore Marker
  • 特异性
    ab124980 recognises Nuclear pore complex protein Nup96 of Nuclear pore complex protein Nup98-Nup96.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NUP98 aa 1600-1700. The immunogen sequence is within Nuclear pore complex protein Nup96 of the Nuclear pore complex protein Nup98-Nup96.
    Database link: P52948

  • 阳性对照
    • HeLa, SH-SY5Y, and Caco-2 cell lysates and Human colon tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 188 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC/IF 1/50 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
    • 疾病相关
      Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
      Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
      Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
      Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
      Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
    • 序列相似性
      Belongs to the nucleoporin GLFG family.
      Contains 1 peptidase S59 domain.
    • 结构域
      Contains G-L-F-G repeats.
    • 翻译后修饰
      Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
    • 细胞定位
      Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 96 kDa nucleoporin antibody
      • 98 kDa nucleoporin antibody
      • ADAR2 antibody
      • ADIR2 antibody
      • GLFG-repeat containing nucleoporin antibody
      • Nuclear pore complex protein Nup96 antibody
      • Nuclear pore complex protein Nup98 Nup96 antibody
      • Nucleoporin 98kD antibody
      • nucleoporin 98kDa antibody
      • Nucleoporin Nup96 antibody
      • Nucleoporin Nup98 antibody
      • NUP196 antibody
      • NUP96 antibody
      • Nup98 antibody
      • Nup98-Nup96 antibody
      • NUP98_HUMAN antibody
      see all

    Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker 图像

    • All lanes : Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : Caco-2 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 188 kDa
    • ab124980, at 1/50 to 1/100, staining NUP98 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)参考文献

    This product has been referenced in:
    • Light WH  et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). Read more (PubMed: 23555195) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (postnatal 60days brain)
    Permeabilization
    Yes - 0.4%triton 30min
    Specification
    postnatal 60days brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Dr. liang zhang

    Verified customer

    提交于 Feb 03 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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