Anti-NUP88抗体(ab79785)

概述

  • 产品名称Anti-NUP88抗体
    参阅全部 NUP88 一抗
  • 描述
    兔多克隆抗体to NUP88
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NUP88.

    (Peptide available as ab90188.)

  • 阳性对照
    • This antibody gave a positive signal in HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab79785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).

靶标

  • 功能Essential component of nuclear pore complex.
  • 组织特异性Ubiquitous.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nuclear pore complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 88 kDa nucleoporin antibody
    • karyoporin antibody
    • Nuclear pore complex protein 88 antibody
    • Nuclear pore complex protein Nup88 antibody
    • Nuclear pore complex protein88 antibody
    • Nucleoporin 88 antibody
    • Nucleoporin 88 kDa antibody
    • Nucleoporin 88kDa antibody
    • Nucleoporin Nup88 antibody
    • Nucleoporin88 antibody
    • Nucleoporin88kDa antibody
    • Nup 84 antibody
    • NUP 88 antibody
    • Nup84 antibody
    • NUP88 antibody
    • NUP88_HUMAN antibody
    • Prei 2 antibody
    • Prei2 antibody
    • Preimplantation protein 2 antibody
    • Preimplantation protein2 antibody
    see all

Anti-NUP88 antibody 图像

  • Anti-NUP88 antibody (ab79785) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 87 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ab79785 (1/200) staining NUP88 in HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highligh the nucleus (red). Please refer to Abreview for further experimental details.

    See Abreview

Anti-NUP88 antibody (ab79785)参考文献

ab79785 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

提交于 Oct 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"