The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Use at 2-5 µg/mg of lysate.
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
Contains 1 RanBD1 domain.
Contains FG repeats.
Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling NUP50 with ab85915 at 1/200 (1µg/ml). Detection: DAB.
Western blot - NUP50 antibody (ab85915)
All lanes : Anti-NUP50 antibody (ab85915) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate. at 50 µg Lane 2 : HeLa whole cell lysate. at 15 µg Lane 3 : HeLa whole cell lysate. at 5 µg
Predicted band size : 50 kDa Observed band size : 50 kDa
Exposure time : 30 seconds
Immunoprecipitation - NUP50 antibody (ab85915)
Detection of NUP50 by Western Blot of Immunprecipitate.
ab85915 at 1µg/ml staining NUP50 in HeLa whole cell lysate immunoprecipitated using ab85915 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.