概述

  • 产品名称Anti-NUP50抗体
    参阅全部 NUP50 一抗
  • 描述
    山羊多克隆抗体to NUP50
  • 特异性This antibody is expected to recognise all three human isoforms.
  • 经测试应用适用于: ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide:

    C-ELHKILLEKKDA

    , corresponding to C terminal amino acids 457-468 of Human NUP50(Peptide available as ab22982.)

  • 阳性对照
    • Jurkat lysate.
  • 常规说明Principal Names – NUP50; NPAP60; NPAP60L; MGC39961; DKFZP564A043; nucleoporin 50kDa; nuclear pore-associated protein 60L Official Gene Symbol - NUP50 GenBank Accession Number – NP_009103; NP_705931; NP_710151 LocusLink ID - 10762 (human); 18141 (mouse) Gene Ontology terms - nuclear pore

性能

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应用

Our Abpromise guarantee covers the use of ab4005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 2.5 - 3.8 µg/ml.
WB Use a concentration of 0.2 - 1 µg/ml. Can be blocked with Human NUP50 peptide (ab22982).

靶标

  • 功能Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
  • 组织特异性Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
  • 序列相似性Contains 1 RanBD1 domain.
  • 结构域Contains FG repeats.
  • 细胞定位Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 50 kDa nucleoporin antibody
    • NPAP60 antibody
    • NPAP60L antibody
    • Nuclear pore associated protein 60L antibody
    • Nuclear pore complex protein Nup50 antibody
    • Nuclear pore-associated protein 60 kDa-like antibody
    • Nucleoporin 50 antibody
    • Nucleoporin 50kDa antibody
    • Nucleoporin Nup50 antibody
    • Nup50 antibody
    • NUP50_HUMAN antibody
    • PRO1146 antibody
    see all

Anti-NUP50 antibody 图像

  • ab4005 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab4005 (3.8µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-NUP50 antibody (ab4005)参考文献

This product has been referenced in:
  • Broggini-Tenzer A  et al. Substrate screening identifies a novel target sequence for the proteasomal activity regulated by ionizing radiation. Proteomics 10:304-14 (2010). WB ; Mouse . Read more (PubMed: 19957288) »
  • González JM  et al. Fast regulation of AP-1 activity through interaction of lamin A/C, ERK1/2, and c-Fos at the nuclear envelope. J Cell Biol 183:653-66 (2008). WB ; Mouse . Read more (PubMed: 19015316) »

See all 4 Publications for this product

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