Anti-NUP188抗体(ab86601)

概述

  • 产品名称Anti-NUP188抗体
    参阅全部 NUP188 一抗
  • 描述
    兔多克隆抗体to NUP188
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 1699 and 1749 of human NUP188 (NP_056169.1)

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 196 kDa (predicted molecular weight: 196 kDa).
IP Use at 10 µg/mg of lysate.

靶标

Anti-NUP188 antibody 图像

  • All lanes : Anti-NUP188 antibody (ab86601) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 196 kDa
    Observed band size : 196 kDa


    Exposure time : 3 minutes
  • Detection of NUP188 by Western Blot of Immunprecipitate.
    ab86601 at 1µg/ml staining NUP188 in HeLa whole cell lysate immunoprecipitated using ab86601 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-NUP188 antibody (ab86601)参考文献

ab86601 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Colorectal cancer cell line DLD1)
Total protein in input 500 µg
Immuno-precipitation step Other - Protein A Dynabeads
Specification Colorectal cancer cell line DLD1
Username

Mr. Ajay Labade

Verified customer

提交于 Jan 31 2017

Application Western blot
Sample Human Cell lysate - whole cell (DLD1 cells)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 20 µg
Specification DLD1 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Ajay Labade

Verified customer

提交于 Jan 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"