Anti-Nup153抗体[R4C8] (ab81463)

概述

  • 产品名称Anti-Nup153抗体[R4C8]
    参阅全部 Nup153 一抗
  • 描述
    大鼠单克隆抗体[R4C8] to Nup153
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    Recombinant fragment, corresponding to amino acids 610-1191 of Rat Nup153, fused to a tag.

  • 表位Amino acids 610-1191 (Zn finger and FG repeats domain).
  • 阳性对照
    • WB: Nuclear membrane fraction of HeLa cells and NIH3T3 cells. ICC/IF: HeLa cells and rat neuron.

性能

应用

Our Abpromise guarantee covers the use of ab81463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 154 kDa.
ICC/IF Use at an assay dependent dilution.

靶标

Anti-Nup153 antibody [R4C8] 图像

  • All lanes : Anti-Nup153 antibody [R4C8] (ab81463)

    Lane 1 : Nuclear membrane fraction from HeLa cells
    Lane 2 : Nuclear membrane fraction from NIH3T3 cells


    Predicted band size : 154 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent staining of HeLa cells with ab81463.
  • Immunofluorescent staining of rat neuron with ab81463. The dots are Nup153.

Anti-Nup153 antibody [R4C8] (ab81463)参考文献

ab81463 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming that information.
I have processed your request to receive ab81463 as ab93310 did not work in western blot.
If there is anything else I can help you with, please let me know.

Thank you for that,
I believe I have found the original order and so I just wanted to confirm the shipping date (just to make sure). Was it ordered on Jan 16th 2012, or about that date? If for any reason you need to contact us about a refund or re...

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Thank you for providing that information and sorry for the confusion about the antibody in question.
I agree that the conditions and quantities you are using should be more than sufficient to get a signal in western blot using ab93310. Therefore I...

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Thank you for your reply.
I am sorry that you are having problems with ab81463 in both western blot and IP, they are covered under our Abpromise to work in both these applications.
However, could you please provide me some more information ab...

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Thank you for contacting Abcam.
I am sorry that you were having trouble opening the webpage for ab81463, please try the link below:
http://www.abcam.com/Nup153-antibody-R4C8-ab81463.html
I have also attached the datasheet for this produc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"