• 产品名称
  • 描述
    兔多克隆抗体to Nup107
  • 宿主
  • 经测试应用
    适用于: ICC/IF, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 20 amino acid peptide from near the center of human Nup107

  • 阳性对照
    • A549 cell lysate.



Our Abpromise guarantee covers the use of ab73290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa).
ICC Use a concentration of 10 µg/ml.


  • 功能
    Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC.
  • 组织特异性
    Ubiquitously expressed in fetal and adult tissues.
  • 疾病相关
    Nephrotic syndrome 11
  • 序列相似性
    Belongs to the nucleoporin Nup84/Nup107 family.
  • 细胞定位
    Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755).
  • Information by UniProt
  • 数据库链接
  • 别名
    • 107 kDa nucleoporin antibody
    • NU107_HUMAN antibody
    • Nuclear pore complex protein Nup107 antibody
    • Nucleoporin 107kDa antibody
    • Nucleoporin Nup107 antibody
    • NUP107 antibody
    • NUP84 antibody
    • NUP84, yeast, homolog of antibody
    • OTTHUMP00000240604 antibody
    • OTTHUMP00000240606 antibody
    see all


  • Lane 1 : Anti-Nup107 antibody (ab73290) at 1 µg/ml
    Lane 2 : Anti-Nup107 antibody (ab73290) at 2 µg/ml

    All lanes : A549 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 106 kDa
    Observed band size: 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 194 kDa. We are unsure as to the identity of these extra bands.

  • ab73290 at 10µg/ml staining Nup107 in A549 cells by Immunocytochemistry.

  • Immunofluorescence of NUP107 in A549 cells using ab73290 at 10 ug/ml.


ab73290 has not yet been referenced specifically in any publications.


Since you are doing mouse on mouse staining, I would recommend either using Fab fragments for blocking, or directly conjugating your primary antibody to a fluorescent secondary. If you want to use the Fab fragment method, I have attached the Abcam prot...

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I am unable to provide tracking information as this antibody has not been shipped yet. Also, as you purchased the antibody from Cedarlane we have to ship the antibody to them first and they will then ship it to you. As I mentioned in the previous email...

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Thank you for your reply. I have processed you request to receive ab24609 as a replacement for ab73290, which did not work as guaranteed in ICC/IF. As this is being shipped to Canada, I am hoping that it will arrive early next week but due to Customs w...

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Thank you for your reply. I found one order from 24th March 2011 that was shipped to the address you gave. Would that date be correct, as in your original email, you said you have ordered these antibodies in the last few months and I just want to make ...

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Thank you for your reply. I would be happy to send you ab24609 as a replacement antibody, but if I remember correctly, you had emailed previously about concerns over this antibodies ability to recognize Nup107. As I said I more than happy to send you t...

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Thank you for providing the protocol information. I was just wandering if you use/or have tried a permeabilization step? I ask as since it is a nuclear protein, it is generally recommended to use a 0.25% Triton step. If you have already tried using the...

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