概述

  • 产品名称Anti-NuMA抗体[EP3976]
    参阅全部 NuMA 一抗
  • 描述
    兔单克隆抗体[EP3976] to NuMA
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • K562, HeLa, MCF7, Raji, SW480, CACO2, and MOLT4 cell lysates; Human breast carcinoma, normal breast and colonic adenocarcinoma tissues; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-NuMA antibody (Alexa Fluor® 488) [EP3976] (ab203993)

    Anti-NuMA antibody (Alexa Fluor® 647) [EP3976] (ab204018)

     

性能

应用

Our Abpromise guarantee covers the use of ab109262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 238 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能May be a structural component of the nucleus.
    • 细胞定位Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
      • NMP 22 antibody
      • Nuclear matrix protein 22 antibody
      • Nuclear mitotic apparatus protein 1 antibody
      • Nuclear mitotic apparatus protein antibody
      • NUMA 1 antibody
      • NUMA antibody
      • NuMA protein antibody
      • NUMA1 antibody
      • NUMA1_HUMAN antibody
      • SP H antigen antibody
      • SP-H antigen antibody
      • Structural nuclear protein antibody
      see all

    Anti-NuMA antibody [EP3976] 图像

    • All lanes : Anti-NuMA antibody [EP3976] (ab109262) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Raji cell lysate
      Lane 5 : SW480 cell lysate
      Lane 6 : CACO2 cell lysate
      Lane 7 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 238 kDa
    • ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast carcinoma by Immunohistochemistry.
    • ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast tissue by Immunohistochemistry.
    • ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human colonic adenocarcinoma by Immunohistochemistry.
    • ab109262, at 1/100 dilution, staining NuMA in HeLa cells by Immunofluorescence.

    Anti-NuMA antibody [EP3976] (ab109262)参考文献

    This product has been referenced in:
    • Green AL  et al. Preclinical antitumor efficacy of selective exportin 1 inhibitors in glioblastoma. Neuro Oncol 17:697-707 (2015). Read more (PubMed: 25366336) »
    • Saare M  et al. Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis. PLoS One 10:e0129131 (2015). ICC ; Mouse . Read more (PubMed: 26062014) »

    See all 3 Publications for this product

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