Recombinant protein fragment corresponding to a region within amino acids 1 and 309 of Human NuMA (AAH04165).
293T, A431, H1299, HeLa, MOLT4 or Raji cell lines.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 238 kDa.
1/100 - 1/500.
1/100 - 1/200.
May be a structural component of the nucleus.
Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
Information by UniProt
Centrophilin stabilizes mitotic spindle in mitotic cells antibody
NMP 22 antibody
Nuclear matrix protein 22 antibody
Western blot - NuMA antibody (ab97585)
Anti-NuMA antibody (ab97585) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 238 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - NuMA antibody (ab97585)
ab97585, at 1/200, staining paraformaldehyde-fixed A431 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NuMA antibody (ab97585)
ab97585, at 1/100, staining paraffin-embedded Cal27 Xenograft by Immunohistochemistry.
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