概述

  • 产品名称Anti-NuMA抗体
    参阅全部 NuMA 一抗
  • 描述
    兔多克隆抗体to NuMA
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 1 and 309 of Human NuMA (AAH04165).

  • 阳性对照
    • 293T, A431, H1299, HeLa, MOLT4 or Raji cell lines.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 238 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能May be a structural component of the nucleus.
  • 细胞定位Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Anti-NuMA antibody 图像

  • Anti-NuMA antibody (ab97585) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 238 kDa
  • ab97585, at 1/200, staining paraformaldehyde-fixed A431 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
  • ab97585, at 1/100, staining paraffin-embedded Cal27 Xenograft by Immunohistochemistry.

Anti-NuMA antibody (ab97585)参考文献

This product has been referenced in:
  • Gruber Filbin M  et al. Coordinate activation of Shh and PI3K signaling in PTEN-deficient glioblastoma: new therapeutic opportunities. Nat Med 19:1518-23 (2013). IHC ; Human . Read more (PubMed: 24076665) »
  • Gregory LS  et al. Breast cancer cells induce osteolytic bone lesions in vivo through a reduction in osteoblast activity in mice. PLoS One 8:e68103 (2013). IHC ; Human . Read more (PubMed: 24069136) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample Human Tissue sections (Engineerd construct)
Specification Engineerd construct
Permeabilization No
Fixative Paraformaldehyde
Username

Mr. Hendrik Gremmels

Verified customer

提交于 Jan 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"